Rowley S D, Brown N C
J Virol. 1977 Feb;21(2):493-6. doi: 10.1128/JVI.21.2.493-496.1977.
The replication of the Bacillus subtilis bacteriophages SPP-1 and phi 105 is sensitive to 6-(p-hydroxyphenylazo)-uracil (HPUra), a selective inhibitor of replicative DNA synthesis of B. subtilis which acts specifically at the levels of a replication-specific polymerase, DNA polymerase III (pol III). The origin of the HPUra-sensitive polymerase required for phage replication was examined by comparison of the drug sensitivity of phage development in a normosensitive host with that in a host carrying azp-12, a polC mutation that specifies production of an HPUra-resistant pol III. azp-12 specified HPUra-resistant phage host pol III. The host polIII requirement for SPP-1 replication also was confirmed by the demonstration that phage development was temperature sensitive in a host mutant carrying the polC mutation mut-1 (ts). Examination of the pol III activity of crude and purified cell-free preparations derived from phage-infected cells did not indicate any detectable changes in the specific activity, purification behavior, or drug sensitivity of the enzyme.
枯草芽孢杆菌噬菌体SPP - 1和phi 105的复制对6 -（对羟基苯偶氮）尿嘧啶（HPUra）敏感，HPUra是枯草芽孢杆菌复制性DNA合成的一种选择性抑制剂，它特异性作用于复制特异性聚合酶——DNA聚合酶III（pol III）水平。通过比较在正常敏感宿主和携带azp - 12（一种指定产生HPUra抗性pol III的polC突变）的宿主中噬菌体发育的药物敏感性，研究了噬菌体复制所需的HPUra敏感聚合酶的来源。azp - 12指定了对HPUra抗性的噬菌体宿主pol III。通过证明在携带polC突变mut - 1（ts）的宿主突变体中噬菌体发育对温度敏感，也证实了宿主polIII对SPP - 1复制的需求。对来自噬菌体感染细胞的粗制和纯化的无细胞制剂的pol III活性进行检测，未发现该酶的比活性、纯化行为或药物敏感性有任何可检测到的变化。
