de Oliveira Fernandes Thaís, de Moura Dalila Ferreira Silvano, Küchler Erika Calvano, Abuabara Allan, Baratto-Filho Flares, de Abreu Fernanda Volpe, Antunes Leonardo Santos, Antunes Lívia Azeredo Alves
Posgraduate Program in Dentistry, Institute of Health Sciences, Fluminense Federal University, Nova Friburgo, RJ, Brazil.
School of Biomedicine, Institute of Health Sciences, Fluminense Federal University, Nova Friburgo, RJ, Brazil.
Biochem Genet. 2025 Apr 9. doi: 10.1007/s10528-025-11092-5.
To investigate the association of polymorphisms in the SOD2 (rs5746136, rs10370, and rs4880) and SOD3 (rs2855262, and rs13306703) genes and dental caries in primary dentition. This cross-sectional study included 753 children aged from 2 to 6 years in primary dentition from 33 public preschools in Nova Friburgo, Rio de Janeiro, Brazil. Covariates such as gender and body mass index were collected. Dental caries experience was evaluated using WHO (2013) criteria. Phenotypes were classified as absence (dmft = 0), presence (dmft ≥ 1) or high caries experience (dmft ≥ 5). Genotyping of the selected polymorphisms was carried out by TaqMan real-time PCR using genomic DNA extracted from buccal cells. Allele and genotype frequencies in recessive, co-dominant and dominant models were compared between phenotype groups. Covariates did not influence on caries experience in any phenotype analyzed. The allele C in rs2855262 (SOD3) presented association with high dental caries experience (dmft ≥ 5) (p = 0.04). The polymorphisms rs5746136 (SOD2) and rs2855262 (SOD3) presented association with caries experience (respectively, dmft ≥ 1 and dmft ≥ 5) in recessive models (p = 0.02). The findings suggest that polymorphisms in SOD2 (rs5746136) and SOD3 (rs2855262) genes are associated with dental caries experience in children with primary dentition. The cross-sectional study design presents known limitations, such as the inability to establish causal relationships. Therefore, the findings of this study should be interpreted with caution, and further is needed to confirm the results and investigate the function of these genes function and their involvement in dental caries and other oral conditions. Altered salivary oxidative stress biomarkers, influenced by genetic variations, play a significant role in the development of dental caries. It emphasizes the importance of genetic screening in oral health assessments. Understanding the SOD2 and SOD3 polymorphisms, could pave the way for personalized preventive strategies and targeted therapeutic interventions in pediatric dentistry.
研究超氧化物歧化酶2（SOD2，rs5746136、rs10370和rs4880）及超氧化物歧化酶3（SOD3，rs2855262和rs13306703）基因多态性与乳牙列龋齿的关联。这项横断面研究纳入了巴西里约热内卢新弗里堡33所公立幼儿园753名2至6岁处于乳牙列期的儿童。收集了性别和体重指数等协变量。采用世界卫生组织（2013年）标准评估龋齿经历。将表型分为无龋齿（dmft = 0）、有龋齿（dmft≥1）或高龋齿经历（dmft≥5）。通过TaqMan实时聚合酶链反应对从颊细胞提取的基因组DNA进行所选多态性的基因分型。比较表型组之间隐性、共显性和显性模型中的等位基因和基因型频率。协变量对任何分析的表型中的龋齿经历均无影响。rs2855262（SOD3）中的等位基因C与高龋齿经历（dmft≥5）相关（p = 0.04）。rs5746136（SOD2）和rs2855262（SOD3）多态性在隐性模型中分别与龋齿经历（dmft≥1和dmft≥5）相关（p = 0.02）。研究结果表明，SOD2（rs5746136）和SOD3（rs2855262）基因多态性与乳牙列期儿童的龋齿经历相关。横断面研究设计存在已知局限性，如无法建立因果关系。因此，本研究结果应谨慎解读，需要进一步研究以证实结果，并调查这些基因的功能及其在龋齿和其他口腔疾病中的作用。受基因变异影响的唾液氧化应激生物标志物改变在龋齿发展中起重要作用。这强调了基因筛查在口腔健康评估中的重要性。了解SOD2和SOD3多态性可为儿童牙科的个性化预防策略和靶向治疗干预铺平道路。
