Kh Abdulrahman Abdulrahman Zainab, Inco Hasan, Ercan Kenan, Aytac Ismail, Taysi Seyithan
Department of Biochemistry and Technology, Graduate School of Natural and Applied Sciences, Gaziantep University, Gaziantep, Turkey.
Department of Otolaryngology, Medical School, Gaziantep University, Gaziantep, Turkey.
Sci Rep. 2025 Apr 26;15(1):14615. doi: 10.1038/s41598-025-95453-5.
Nasal polyposis (NP), a chronic inflammatory disease of the upper respiratory tract, has a poorly understood pathophysiology and no etiological factors explaining its pathogenesis, but inflammation remains an important factor. Sestrins (SESNS), which are conserved antioxidant proteins that accumulate in cells in response to various stresses, regulate the regulatory roles of SESN2 in the immune system and inflammatory responses. In the current study, we aimed to investigate the sestrin-2 pathway, oxidative stress and thiol-disulfide homeostasis in NP tissue of patients. The study included a total of 90 participants with the age 12-65 years. The patient group consisted of 45 patients diagnosed with NP and free from chronic disease. The control group comprised 45 patients who underwent nose surgery for anatomical pathology other than NP. The levels of native thiol, total thiol, disulfide, disulfide/native thiol and disulfide/total thiol were found to be significantly higher in the patient group, while native thiol/total thiol levels were lower when compared to the control group. Besides, the levels of total oxidant status (TOS) and oxidative stress index (OSI) were significantly higher while total antioxidant status (TAS) levels were significantly lower compared to the control group. Also, extracellular signal-regulated kinase 1/2 (ERK1/2) levels were found to be higher and SESN2 lower than those in control group. The findings show that decreased SESN2, TAS levels and increased ERK1/2, TOS and OSI levels and impaired thiol-disulfide homeostasis in polyp tissues of patients with NP cause oxidative stress.
鼻息肉病（NP）是上呼吸道的一种慢性炎症性疾病，其病理生理学尚未完全明确，也没有病因学因素能解释其发病机制，但炎症仍是一个重要因素。Sestrins（SESNS）是保守的抗氧化蛋白，在细胞受到各种应激时会积累，它调节SESN2在免疫系统和炎症反应中的调节作用。在本研究中，我们旨在调查NP患者鼻组织中的sestrin-2通路、氧化应激和硫醇-二硫键稳态。该研究共纳入90名年龄在12至65岁之间的参与者。患者组由45名被诊断为NP且无慢性病的患者组成。对照组由45名因非NP的解剖病理学原因接受鼻手术的患者组成。发现患者组中天然硫醇、总硫醇、二硫键、二硫键/天然硫醇和二硫键/总硫醇的水平显著更高，而与对照组相比，天然硫醇/总硫醇水平更低。此外，与对照组相比，总氧化剂状态（TOS）和氧化应激指数（OSI）水平显著更高，而总抗氧化剂状态（TAS）水平显著更低。同时，发现细胞外信号调节激酶1/2（ERK1/2）水平高于对照组，而SESN2水平低于对照组。研究结果表明，NP患者息肉组织中SESN2水平降低、TAS水平降低、ERK1/2、TOS和OSI水平升高以及硫醇-二硫键稳态受损会导致氧化应激。
