Comparative Mitogenomic Analyses of (Diptera: Chironomidae).

, a genus within the species-rich subfamily Orthocladiinae (Diptera: Chironomidae), remains poorly resolved in molecular phylogenetics due to limited available molecular data. Here, we sequenced and analyzed the complete mitogenomes of five species, using two species as outgroups. Our results reveal that the mitogenomes of are structurally conserved and retain a presumed ancestral gene order. The nucleotide composition of these newly generated mitogenomes exhibits a pronounced A + T bias, which is characteristic of typical insect mitogenomes. The substitution rates, estimated using Ka/Ks ratios, indicate that all protein-coding genes are under purifying selection. The strongest purifying selection pressure was observed in the gene, while the weakest was in the gene. Both the maximum likelihood and Bayesian inference trees consistently show the following topology: (((( + ) + ) + ) + ). This study provides key insights into chironomid mitogenomes and their gene properties, offering valuable reference data for future research.