Honig Nele, Teubert Christina, Lamparter Lucas, Keller Marius N, Austermann Judith, Berger Philipp, Schmitz Anne, Rasch Christiane, Nüsse Harald, Klingauf Jürgen, Erpenbeck Luise, Roth Johannes, Galic Milos
Institute of Medical Physics and Biophysics, Medical Faculty, University of Münster, Münster 48149, Germany.
'Cells in Motion' Interfaculty Centre, University of Münster, Münster 48149, Germany.
Biol Open. 2025 May 15;14(5). doi: 10.1242/bio.061704. Epub 2025 May 13.
Neutrophils and monocytes are sentinels of inflammatory signals. To reach the sites of action, both cell types attach to and then transmigrate the endothelial cell layer that lines the luminal side of blood vessels. While it has been reported that neutrophils and monocytes actively migrate along the surface of the vasculature, it remains elusive whether and how these motion patterns augment the efficiency of the immune system. Here, we conducted co-culture experiments of primary human monocytes and neutrophils, respectively, with primary human umbilical vein endothelial cells (HUVECs). Combining classical biomedical approaches with quantitative image analysis and numerical models, we find that immune cells simultaneously increase the number of sampled cells versus traveled distance and sensitivity to chemokines by migrating along endothelial cell-cell boundaries. Collectively, these findings establish search optimization of neutrophils and monocytes through limitation of motion pattern to cell-cell boundaries.
中性粒细胞和单核细胞是炎症信号的哨兵。为了到达作用部位，这两种细胞类型都会附着于血管腔面的内皮细胞层，然后穿越该层。虽然有报道称中性粒细胞和单核细胞会沿着脉管系统表面主动迁移，但这些运动模式是否以及如何提高免疫系统的效率仍不清楚。在这里，我们分别将原代人单核细胞和中性粒细胞与原代人脐静脉内皮细胞（HUVECs）进行共培养实验。结合经典生物医学方法、定量图像分析和数值模型，我们发现免疫细胞通过沿着内皮细胞-细胞边界迁移，同时增加了采样细胞数量与移动距离的比值以及对趋化因子的敏感性。总体而言，这些发现通过将运动模式限制在细胞-细胞边界，确立了中性粒细胞和单核细胞的搜索优化机制。
