Cao Yiwen, Yang Yating, Tan Zhengwei, Feng Xihan, Tian Zhiyao, Liu Tianheng, Pan Yonghui, Wang Min, Su Xiaoyu, Liang Huizhen, Guo Shiwei
Jiangsu Provincial Key Laboratory for Solid Organic Waste Utilization, Key Laboratory of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Institute of Chinese Herbal Medicines, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Int J Mol Sci. 2025 May 7;26(9):4464. doi: 10.3390/ijms26094464.
Mineral nutrition is of vital importance in plant growth and secondary metabolites accumulation, and thereby in the nutritional value of plants. In , a preference to nitrate (NO-N) in comparison to ammonium (NH-N) was found in our previous study, which can be revealed from the rapid growth rate of under NO-N. This study assessed whether a preference for nitrogen sources could invoke metabolic reprogramming and interrelationships between factors. NO-fed plants exhibited substantial enhancement of carbon stimulation, which was strongly and positively correlated with mesophyll conductance. As a result, the elevated carbon flux by NO supplement was shuttled to phenolic metabolites synthesis, including flavones and caffeoylquinic acids compounds. Notably, the stimulation was triggered by changes in the NO and C/N ratio and was mediated by the induction of several enzymes in the phenylpropanoid pathway. On the contrary, NH plants showed an increment in the content of nitrogen, carbohydrates, and amino acids (mainly a strong increase in citrulline and theanine). Within secondary metabolism, NH may involve active lignin metabolism, showing a dramatic increment in hydroxy-ferulic acid and lignin content. This work provides significant insights regarding the mechanisms of in response to diverse nitrogen regimes and effective strategies of nitrogen fertilizer input for .
矿物质营养对植物生长和次生代谢产物积累至关重要，从而对植物的营养价值也至关重要。在我们之前的研究中发现，与铵态氮（NH-N）相比，植物更偏好硝态氮（NO-N），这可以从在NO-N条件下[植物名称]的快速生长速率中看出。本研究评估了对氮源的偏好是否会引发代谢重编程以及各因素之间的相互关系。供应NO的植物表现出碳同化的显著增强，这与叶肉导度呈强烈正相关。结果，通过补充NO提高的碳通量被用于酚类代谢产物的合成，包括黄酮类和咖啡酰奎宁酸类化合物。值得注意的是，这种刺激是由NO和C/N比的变化引发的，并由苯丙烷途径中几种酶的诱导介导。相反，供应NH的植物在氮、碳水化合物和氨基酸含量上有所增加（主要是瓜氨酸和茶氨酸大幅增加）。在次生代谢中，NH可能涉及活跃的木质素代谢，表现为羟基阿魏酸和木质素含量显著增加。这项工作为[植物名称]响应不同氮素供应的机制以及[植物名称]氮肥投入的有效策略提供了重要见解。
