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探索微等离子体在控制……中细胞衰老方面的作用

Exploring the Role of Microplasma for Controlling Cellular Senescence in .

作者信息

Begum Farhana, Kristof Jaroslav, Alam Md Jahangir, Sadiq Abubakar Hamza, Hasan Mahedi, Soichiro Kinoshita, Shimizu Kazuo

机构信息

Graduate School of Medical Photonics, Shizuoka University, Hamamatsu 832-8561, Japan.

Graduate School of Science and Technology, Shizuoka University, Hamamatsu 432-8561, Japan.

出版信息

Molecules. 2025 Apr 29;30(9):1970. doi: 10.3390/molecules30091970.

DOI:10.3390/molecules30091970
PMID:40363776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12073758/
Abstract

Cellular senescence plays a pivotal role in aging and stress response mechanisms. Controlling cellular senescence is essential for developing novel techniques to prevent aging or aging-related diseases and promote a healthy lifespan. This study explores the efficiency of cold atmospheric microplasma (CAM) for controlling cellular senescence in yeast . Reactive oxygen and nitrogen species (RONS) generated by CAM influence key processes, such as the regulation of oxidative stress, alterations in membrane potential, and senescence-related epigenetic modifications. As a marker of cellular senescence, the expression of β-galactosidase was assessed in response to different plasma treatments. At a frequency of 1 kHz and a discharge voltage of 5 kV, a significant reduction in β-galactosidase activity was observed in cells treated for 10 s and 30 s compared to the control, indicating a reduction in cellular senescence. Additionally, cell viability, metabolic activity, and plasma membrane potential were also found to be higher for the treated cells compared to the control under the same conditions. This study confirms that a physiologically tolerable level of ROS and RNS is sufficient for cellular signaling, but not for damage induction. The findings from this study provide insights on the potential of microplasma as a tool for controlling cellular senescence and the development of therapeutic innovations involving eukaryotic cells.

摘要

细胞衰老在衰老和应激反应机制中起着关键作用。控制细胞衰老对于开发预防衰老或与衰老相关疾病的新技术以及促进健康寿命至关重要。本研究探讨了冷大气微等离子体（CAM）控制酵母细胞衰老的效率。CAM产生的活性氧和氮物种（RONS）影响关键过程，如氧化应激调节、膜电位改变和衰老相关的表观遗传修饰。作为细胞衰老的标志物，评估了β-半乳糖苷酶在不同等离子体处理后的表达。在1kHz频率和5kV放电电压下，与对照相比，处理10秒和30秒的细胞中β-半乳糖苷酶活性显著降低，表明细胞衰老减少。此外，在相同条件下，与对照相比，处理后的细胞的细胞活力、代谢活性和质膜电位也更高。本研究证实，生理上可耐受水平的ROS和RNS足以用于细胞信号传导，但不足以诱导损伤。本研究结果为微等离子体作为控制细胞衰老的工具的潜力以及涉及真核细胞的治疗创新的发展提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2153/12073758/81304ffce61c/molecules-30-01970-g015.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2153/12073758/58f232e0a0e9/molecules-30-01970-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2153/12073758/14ff6b36c778/molecules-30-01970-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2153/12073758/585ac7a3c5c0/molecules-30-01970-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2153/12073758/c718222655bb/molecules-30-01970-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2153/12073758/32965fe54f90/molecules-30-01970-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2153/12073758/81304ffce61c/molecules-30-01970-g015.jpg

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