Walz D
Food Chem Toxicol. 1985 Feb;23(2):199-203. doi: 10.1016/0278-6915(85)90017-1.
Intracutaneous injection of aqueous solutions of topically irritant substances into the skin of juvenile mice creates a persisting oedema, the strength of which can be assessed by the number of wrinkles formed on reefing the skin at the site of injection. An algorithm is used to transform the number of wrinkles, recorded just before and 2.5 and 6 hr after the injection, into the intensity of the oedematous reaction. This quantity, which does not depend on subjective assessments graded on an arbitrary scale, is reproducible and has been judged to be relevant to the prediction of irritancy likely to arise from accidental contact of substances with mucous membranes.
将局部刺激性物质的水溶液皮内注射到幼年小鼠的皮肤中会产生持续的水肿，水肿的程度可通过注射部位皮肤折叠时形成的皱纹数量来评估。使用一种算法将注射前、注射后2.5小时和6小时记录的皱纹数量转化为水肿反应的强度。这个量不依赖于基于任意量表分级的主观评估，具有可重复性，并且已被判定与预测物质意外接触粘膜可能产生的刺激性相关。
