Bucher K, Bucher K E, Walz D
Agents Actions. 1979 Apr;9(1):124-32. doi: 10.1007/BF02024143.
The abdominal skin of juvenile white mice was used. Topical application was accomplished by intracutaneous injection. The solutions tested were thoroughly standardized. By using a special buffer system, a mixture of histidine glutamate and lysine glutamate, each pH value could be tested with the same buffer capacity. The pH values varied from pH 3 to pH 11, the solutions being always iso-osmolalic with plasma. Two criteria were used to judge the irritancy: (a) the edematous reaction for the first 6 h and (b) the macroscopic aspect after 24 h. A very special procedure enabled us to obtain objective numerical values for criterion (a). The results disclose that irritation becomes manifest at pH 4 and pH 10, becomes clear-cut with pH 3 and even more so with pH 11, and depends strongly on the buffer capacity employed. With two substances-amidosulfuric acid and (tri) sodium phosphate--it was exemplified how the procedure could be used to routinely screen for topical irritancy in an informative, inexpensive and decent manner.
使用幼年白鼠的腹部皮肤。通过皮内注射进行局部应用。所测试的溶液经过了充分标准化。通过使用一种特殊的缓冲系统，即组氨酸谷氨酸和赖氨酸谷氨酸的混合物，每种pH值都可以在相同的缓冲容量下进行测试。pH值范围为pH 3至pH 11，溶液始终与血浆等渗。使用两个标准来判断刺激性：(a) 最初6小时的水肿反应和(b) 24小时后的宏观外观。一种非常特殊的程序使我们能够获得标准(a)的客观数值。结果表明，在pH 4和pH 10时刺激变得明显，在pH 3时变得清晰，在pH 11时更是如此，并且强烈依赖于所使用的缓冲容量。以氨基磺酸和磷酸（三）钠这两种物质为例，说明了该程序如何能够以一种信息丰富、成本低廉且合适的方式常规筛选局部刺激性。
