Pitol-Palin Letícia, Sousa Isadora Castaldi, de Araújo Juliani Caroline Ribeiro, de Souza Batista Fábio Roberto, Inoue Bruna Kaori Namba, Botacin Paulo Roberto, de Vasconcellos Luana Marotta Reis, Lisboa-Filho Paulo Noronha, Okamoto Roberta
Department of Diagnosis and Surgery, Araçatuba Dental School, São Paulo State University, Araçatuba 16015-050, Brazil.
Department of Basic Sciences, Araçatuba Dental School, São Paulo State University, Araçatuba 16066-840, Brazil.
Biology (Basel). 2025 Apr 26;14(5):476. doi: 10.3390/biology14050476.
Dental rehabilitation with titanium implants may requires the optimization of techniques and materials when oral conditions affect the successful treatment result. Thus, this study aims to customize the surface of titanium implants with bioactive vitamin D3 molecules to increase the performance of bone repair. The surfaces were functionalized following the "dip-coating" incorporation method with vitamin D3 in a solution of 1000 I.U./goat. The work was carried out in two stages: (I) physicochemical and biological tests (in vivo) in order to characterize and validate the vitamin D3 surface as well as its ability to affect peri-implant bone biomechanics; and (II) in vitro experiments to characterize viability responses, interaction and cell mineralization capacity. Scanning electron microscopy showed that the creation of vitamin D3 films is stable and homogeneous, while the in vivo results showed an increase in the biomechanical and microarchitectural capacity of the bone when vitamin D3 implants were used. Furthermore, the application of functionalized surfaces proved effective in promoting cell interaction and bone mineralization processes while preserving cell viability and capacity. In conclusion, the delivery of bioactive molecules based on vitamin D3 promotes changes in the surface microstructure of titanium, enabling an increase in the structural characteristics of bone tissue that result in an improvement in bone repair and peri-implant biomechanics.
当口腔状况影响治疗效果时，使用钛植入物进行牙齿修复可能需要优化技术和材料。因此，本研究旨在用生物活性维生素D3分子定制钛植入物表面，以提高骨修复性能。采用“浸涂”法将维生素D3以1000国际单位/克的溶液掺入，对表面进行功能化处理。这项工作分两个阶段进行：（I）物理化学和生物学测试（体内），以表征和验证维生素D3表面及其影响种植体周围骨生物力学的能力；（II）体外实验，以表征活力反应、相互作用和细胞矿化能力。扫描电子显微镜显示，维生素D3膜的形成稳定且均匀，而体内结果显示，使用维生素D3植入物时，骨的生物力学和微观结构能力有所提高。此外，功能化表面的应用在促进细胞相互作用和骨矿化过程方面被证明是有效的，同时保留了细胞活力和能力。总之，基于维生素D3的生物活性分子的递送促进了钛表面微观结构的变化，能够增加骨组织的结构特征，从而改善骨修复和种植体周围生物力学。
