Morgan D R, Johnson P C, DuPont H L, Satterwhite T K, Wood L V
Infect Immun. 1985 Oct;50(1):62-5. doi: 10.1128/iai.50.1.62-65.1985.
Five strains of Aeromonas hydrophila were selected for use in volunteer challenge trials. All five strains produced cytotoxin, hemolysin enterotoxin, lysine decarboxylase, acetylmethylcarbinol, and DNase. Two strains hydrolyzed esculin. All strains produced purulent hemorrhagic fluid accumulation in rabbit ileal loops, but failed to induce keratoconjunctivitis in guinea pigs. None of the strains produced mannose-resistant hemagglutinins. In challenge studies, diarrhea was demonstrated in only 2 of 57 human volunteers with doses ranging from 10(4) to 10(10) CFU. One person experienced mild diarrhea with 10(9) CFU of strain 6Y. A second person developed moderate diarrhea with 10(7) CFU of strain 3647. At higher doses, no diarrhea was seen in any of the volunteers. The other three strains (B158, SSU, 3284) failed to cause diarrhea and were not recovered from stools of volunteers. Additional virulence properties of A. hydrophila need to be sought before enteropathogenicity for humans can be established.
选取了5株嗜水气单胞菌用于志愿者激发试验。所有5株菌均产生细胞毒素、溶血素肠毒素、赖氨酸脱羧酶、乙酰甲基甲醇和DNA酶。2株菌能水解七叶苷。所有菌株均可使兔回肠袢产生脓性出血性积液，但未能在豚鼠中诱发角膜结膜炎。所有菌株均不产生甘露糖抗性血凝素。在激发研究中，57名人类志愿者中只有2人出现腹泻，剂量范围为10⁴至10¹⁰CFU。一人摄入10⁹CFU的6Y菌株后出现轻度腹泻。另一人摄入10⁷CFU的3647菌株后出现中度腹泻。在更高剂量下，任何志愿者均未出现腹泻。其他三株菌（B158、SSU、3284）未引起腹泻，且未从志愿者粪便中分离出来。在确定嗜水气单胞菌对人类的肠道致病性之前，需要寻找其更多的毒力特性。
