Goida Anastasia, Krasnova Tatiana, Shamagsumova Rezeda, Evtugyn Vladimir, Saveliev Anatoly, Porfireva Anna
A.M. Butlerov's Chemistry Institute, Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia.
Interdisciplinary Center of Analytical Microscopy, Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia.
Biosensors (Basel). 2025 Jun 14;15(6):385. doi: 10.3390/bios15060385.
A novel nanocomposite based on electrochemically reduced graphene oxide (ERGO) and electropolymerized polyproflavine (PPFL) was obtained within a "one-pot" synthesis from natural deep eutectic solvent (NADES). NADES consisted of citric acid, glucose, and water in a molar ratio of 1:1:6. The synthesis was carried out in potentiostatic mode by consequent potential application in cathodic and anodic areas. The composite was applied to develop the impedimetric DNA sensor for anthracycline determination. The sensor has provided linear range from 10 nM to 0.1 mM for doxorubicin, from 1 pM to 10 nM for epirubicin, and from 10 pM to 10 nM for idarubicin, with the limit of detection 3 nM, 1 pM, and 5 pM, respectively. The concentrations of doxorubicin below 10 nM did not have any other influence on epirubicin and idarubicin determination despite their molecular structure similarity. The sensor developed was used for the determination of anticancer medications, such as doxorubicin, epirubicin, and idarubicin, in their standard solutions, pharmaceuticals, artificial, and human urine samples. It is worth noting that the additions of mannitol and lactose, which are the stabilizers of the pharmaceuticals, exhibited an interfering effect on the sensor response.
通过在天然低共熔溶剂（NADES）中进行“一锅法”合成，获得了一种基于电化学还原氧化石墨烯（ERGO）和电聚合聚原黄素（PPFL）的新型纳米复合材料。NADES由柠檬酸、葡萄糖和水按1:1:6的摩尔比组成。合成过程通过在阴极和阳极区域依次施加电位以恒电位模式进行。该复合材料被用于开发用于测定蒽环类药物的阻抗型DNA传感器。对于阿霉素，该传感器的线性范围为10 nM至0.1 mM；对于表柔比星，为1 pM至10 nM；对于伊达比星，为10 pM至10 nM，检测限分别为3 nM、1 pM和5 pM。尽管阿霉素、表柔比星和伊达比星的分子结构相似，但低于10 nM的阿霉素浓度对表柔比星和伊达比星的测定没有任何其他影响。所开发的传感器用于测定标准溶液、药物、人工尿液和人体尿液样本中的抗癌药物，如阿霉素、表柔比星和伊达比星。值得注意的是，药物稳定剂甘露醇和乳糖的添加对传感器响应表现出干扰作用。
