公共卫生实验室服务处用于检测风疹特异性 IgM 的 IgM 抗体捕获酶联免疫吸附测定法。
Public Health Laboratory Service IgM antibody capture enzyme linked immunosorbent assay for detecting rubella specific IgM.
作者信息
Bellamy K, Hodgson J, Gardner P S, Morgan-Capner P
出版信息
J Clin Pathol. 1985 Oct;38(10):1150-4. doi: 10.1136/jcp.38.10.1150.
A total of 468 sera were selected for the evaluation of the Public Health Laboratory Service's IgM antibody capture enzyme linked immunosorbent assay kit (MACELISA) for detecting rubella specific IgM. The results obtained were compared with those obtained by IgM antibody capture radioimmunoassay (MACRIA). Sera from patients with primary postnatal rubella, congenital rubella, remote rubella, infectious mononucleosis, and recent infection with other agents were included, in addition to sera taken after rubella immunisation and sera containing rheumatoid factor and rubella specific IgG antibody. The assay exhibited a similar ability and comparable specificity to MACRIA for detecting rubella specific IgM antibody. The Public Health Laboratory Service MACELISA can be recommended if, as for all assays that detect rubella specific IgM, all the available clinical and serological data are taken into account when the results are interpreted.
共选取468份血清，用于评估公共卫生实验室服务中心检测风疹特异性IgM的IgM抗体捕获酶联免疫吸附测定试剂盒（MACELISA）。将所得结果与IgM抗体捕获放射免疫测定法（MACRIA）的结果进行比较。除了风疹免疫接种后采集的血清以及含有类风湿因子和风疹特异性IgG抗体的血清外，还纳入了原发性产后风疹、先天性风疹、远期风疹、传染性单核细胞增多症患者以及近期感染其他病原体患者的血清。该检测方法在检测风疹特异性IgM抗体方面表现出与MACRIA相似的能力和相当的特异性。如果像所有检测风疹特异性IgM的检测方法一样，在解释结果时考虑到所有可用的临床和血清学数据，那么公共卫生实验室服务中心的MACELISA是可以推荐使用的。
Zotero 插件