使用Sephacryl S-300凝胶过滤法检测风疹特异性IgM。
Rubella-specific IgM detection using Sephacryl S-300 gel filtration.
作者信息
Morgan-Capner P, Davies E, Pattison J R
出版信息
J Clin Pathol. 1980 Nov;33(11):1082-5. doi: 10.1136/jcp.33.11.1082.
Sephacryl S-300 has been compared with Sephadex G-200 in the fractionation of sera for the detection of rubella-specific IgM. No difference in sensitivity was found with sera that had low titres of rubella-specific IgM. Sephadex G-200 was apparently more sensitive with some sera which had high titres of rubella-specific IgM due to the contribution of rubella-specific IgA. Sephacryl S-300 offers considerable advantages in ease and speed of preparation, and as it is a more rigid gel higher flow rates can be obtained. Sera may be fractionated in 2.5 hours on Sephacryl S-300 compared with 7-10 hours on Sephadex G-200.
在用于检测风疹特异性 IgM 的血清分级分离中，已将 Sephacryl S - 300 与 Sephadex G - 200 进行了比较。对于风疹特异性 IgM 滴度较低的血清，未发现敏感性有差异。由于风疹特异性 IgA 的作用，Sephadex G - 200 对一些风疹特异性 IgM 滴度较高的血清显然更敏感。Sephacryl S - 300 在制备的简便性和速度方面具有相当大的优势，并且由于它是一种更坚硬的凝胶，可以获得更高的流速。与在 Sephadex G - 200 上分级分离血清需要 7 - 10 小时相比，在 Sephacryl S - 300 上血清分级分离可在 2.5 小时内完成。
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