Sexton S A, Hodgson J, Morgan-Capner P
J Hyg (Lond). 1982 Jun;88(3):453-61. doi: 10.1017/s0022172400070315.
An immunosorbent assay using solid-phase attachment of red cells (SPARC) was used for the detection of rubella-specific IgM. The method is described and the results compared with those obtained by the IgM antibody capture radioimmunoassay (MACRIA). One hundred and ninety-nine sera were investigated for the presence of rubella-specific IgM and only one discrepant result occurred, namely a false positive obtained by MACRIA in a patient with infectious mononucleosis. Rheumatoid factor, heterophile antibody, and rubella-specific IgG did not interfere with the results obtained by the SPARC technique. Advantages of the SPARC technique include the case and lack of expense of testing large numbers of sera, the small volume of sample required and the fact that pretreatment of serum is not necessary.
采用红细胞固相吸附免疫吸附测定法（SPARC）检测风疹特异性IgM。本文描述了该方法，并将结果与IgM抗体捕获放射免疫测定法（MACRIA）的结果进行了比较。对199份血清进行了风疹特异性IgM检测，仅出现1例结果不一致的情况，即在1例传染性单核细胞增多症患者中MACRIA检测出现假阳性。类风湿因子、嗜异性抗体和风疹特异性IgG均不干扰SPARC技术获得的结果。SPARC技术的优点包括检测大量血清简便且成本低、所需样本量小以及无需对血清进行预处理。
