Ibáñez-Escribano Alexandra, Nogal-Ruiz Juan José, Escario José Antonio, Ponce-Gordo Francisco
Department of Microbiology and Parasitology, Faculty of Pharmacy, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Biology (Basel). 2025 May 27;14(6):618. doi: 10.3390/biology14060618.
causes one of the most prevalent sexually transmitted infections (STIs). Despite extensive biomolecular research on this protozoan, no efficient molecular tool currently exists for the intraspecific classification of isolates. In recent years, an incipient tendency has been observed to classify this parasite into two genotypes that correlate in a high percentage with phenotypic characteristics (such as metronidazole resistance and viral endobionts). However, this grouping method has not yet been consolidated by the scientific community. In this sense, has also been identified as an endobiont of , but no previous studies on its presence and possible correlation with the two populations detected have been executed. In the present study, several new isolates of Spanish origin have been characterized using two molecular markers (microsatellites and single-copy genes), the presence of virus (TVV) and , and the resistance to reference drugs. On the basis of our molecular results, the isolates were classified into two groups, as proposed by other researchers. In relation to the biological determinations, the two isolates harboring were sensible to the reference drugs and were included in the same genotypic group. Although the presence of TVV was more notable in one group than in the other, both groups had TTV+ and TVV- isolates. These findings indicate that genetic grouping does not strongly correlate with susceptibility to reference drugs or endobiont presence, suggesting the need for further research into alternative classification models.
引起最常见的性传播感染（STIs）之一。尽管对这种原生动物进行了广泛的生物分子研究，但目前尚无有效的分子工具用于分离株的种内分类。近年来，已观察到一种初步趋势，即将这种寄生虫分为两种基因型，这与表型特征（如甲硝唑耐药性和病毒内共生体）有很高的相关性。然而，这种分组方法尚未得到科学界的巩固。从这个意义上说， 也被确定为 的内共生体，但之前尚未对其存在以及与检测到的两种 群体的可能相关性进行研究。在本研究中，使用两种分子标记（微卫星和单拷贝基因）、 病毒（TVV）的存在情况以及 ，并结合对参考药物的耐药性，对几个西班牙来源的新 分离株进行了表征。根据我们的分子结果，这些分离株按照其他研究人员的提议被分为两组。关于生物学测定，携带 的两个分离株对参考药物敏感，并被归入同一基因型组。尽管TVV在一组中的存在比另一组更显著，但两组都有TTV +和TVV -分离株。这些发现表明，基因分组与对参考药物的敏感性或内共生体的存在没有强烈的相关性，这表明需要进一步研究替代分类模型。
