Leibl H, Stingl G, Pehamberger H, Korschan H, Konrad K, Wolff K
J Invest Dermatol. 1985 Nov;85(5):417-22. doi: 10.1111/1523-1747.ep12277084.
Azelaic acid was successfully used in the clinical treatment of 7 cases of lentigo maligna in that remission of the lesions was observed in all our patients. In order to elucidate mechanism(s) of the beneficial clinical effects, we studied the effect of azelaic acid on cultured melanoma cells. Cell numbers recovered from melanoma cell cultures grown for several days in the presence of 10 mM azelaic acid were 50-70% less than those recovered from control cultures or from cultures containing 10 mM adipic acid. This reduction of cell numbers was not due to a simple cytotoxic or cytolytic effect of azelaic acid but rather due to a dose-dependent inhibition of DNA synthesis. Interestingly, nontoxic concentrations of azelaic acid, which significantly reduced DNA synthesis of cultured melanoma cells, had no overt effect on the protein synthesis of these cells. It is conceivable that inhibition of DNA synthesis is one of the mechanisms by which azelaic acid prevents growth and proliferation of abnormal melanocytes.
壬二酸已成功用于7例恶性雀斑样痣的临床治疗，所有患者的皮损均有缓解。为阐明其有益临床效果的机制，我们研究了壬二酸对培养的黑色素瘤细胞的作用。在含有10 mM壬二酸的条件下培养数天的黑色素瘤细胞培养物中回收的细胞数量比对照培养物或含有10 mM己二酸的培养物中回收的细胞数量少50-70%。细胞数量的减少不是由于壬二酸的简单细胞毒性或细胞溶解作用，而是由于对DNA合成的剂量依赖性抑制。有趣的是，显著降低培养的黑色素瘤细胞DNA合成的无毒浓度的壬二酸对这些细胞的蛋白质合成没有明显影响。可以想象，DNA合成的抑制是壬二酸阻止异常黑素细胞生长和增殖的机制之一。
