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基于金属-绝缘体-金属环形谐振器的用于检测多种细菌病原体的表面等离子体光学生物传感器的设计

Design of a plasmonic optical biosensor based on a metal-insulator-metal ring resonator for the detection of various bacterial pathogens.

作者信息

Khodaie Ali, Rafighirani Yousef, Heidarzadeh Hamid, Javidan Javad

机构信息

Department of Electrical and Computer Engineering, University of Mohaghegh Ardabili, Ardabil, Iran.

出版信息

Sci Rep. 2025 Jul 1;15(1):20699. doi: 10.1038/s41598-025-07331-9.

Abstract

Rapid and sensitive detection of pathogenic bacteria is essential for healthcare, food safety, and environmental monitoring. However, conventional detection techniques often fall short in terms of the speed and sensitivity required for real-time applications. In this study, we propose a label-free plasmonic optical biosensor based on a metal-insulator-metal (MIM) dual-ring resonator structure for the efficient detection of bacterial species. The sensor geometry was optimized using Particle Swarm Optimization (PSO) and evaluated through three-dimensional finite-difference time-domain (3D-FDTD) simulations to enhance both sensitivity and figure of merit (FOM). Gold nanorings integrated with a gold back reflector were employed due to their superior plasmonic resonance characteristics. The optimized design achieved a sensitivity of 324.76 nm·RIU, a FOM of 10.187 RIU, and a detection limit (LoD) of 0.075 RIU. The biosensor maintained high performance under varying operational conditions, including temperature (0-500 K), incident angle (0°-50°), and polarization states. Strong field confinement in the dielectric gap significantly enhanced the interaction between light and the analyte. The device demonstrated the ability to detect and differentiate between Vibrio cholerae (n = 1.365), Escherichia coli (n = 1.388), and Pseudomonas species (n = 1.437-1.526), highlighting its potential for quantitative bacterial identification. By addressing key limitations in sensitivity and specificity in complex biological environments, this MIM-based sensor offers a robust platform for rapid, high-throughput bacterial detection in clinical diagnostics and beyond.

摘要

快速灵敏地检测病原菌对于医疗保健、食品安全和环境监测至关重要。然而,传统检测技术在实时应用所需的速度和灵敏度方面往往存在不足。在本研究中,我们提出了一种基于金属 - 绝缘体 - 金属(MIM)双环谐振器结构的无标记等离子体光学生物传感器,用于高效检测细菌种类。使用粒子群优化算法(PSO)对传感器几何结构进行了优化,并通过三维时域有限差分法(3D - FDTD)模拟进行评估,以提高灵敏度和品质因数(FOM)。由于其优异的等离子体共振特性,采用了集成金背反射器的金纳米环。优化后的设计实现了324.76 nm·RIU的灵敏度、10.187 RIU的品质因数和0.075 RIU的检测限(LoD)。该生物传感器在包括温度(0 - 500 K)、入射角(0° - 50°)和偏振态在内的不同操作条件下均保持高性能。介电间隙中的强场限制显著增强了光与分析物之间的相互作用。该器件展示了检测和区分霍乱弧菌(n = 1.365)、大肠杆菌(n = 1.388)和假单胞菌属(n = 1.437 - 1.526)的能力,突出了其在定量细菌鉴定方面的潜力。通过解决复杂生物环境中灵敏度和特异性的关键限制,这种基于MIM的传感器为临床诊断及其他领域的快速、高通量细菌检测提供了一个强大的平台。

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