Liu S, Birch C, Coulepis A, Gust I
J Clin Microbiol. 1984 Sep;20(3):347-50. doi: 10.1128/jcm.20.3.347-350.1984.
A radioimmunofocus assay suitable for quantitation of cell culture-adapted human rotavirus was developed. The method was reproducible, more sensitive than plaque assay, and useful to detect and quantify strains of rotavirus which do not produce plaques. Preliminary results also suggested that the technique will be a useful means of serotyping cell culture-adapted strains of the virus.
开发了一种适用于定量细胞培养适应型人轮状病毒的放射免疫聚焦测定法。该方法具有可重复性,比空斑试验更灵敏,可用于检测和定量不产生空斑的轮状病毒株。初步结果还表明,该技术将是对细胞培养适应型病毒株进行血清分型的有用手段。