Lee Dong Jae, Choi Young-Joon
Department of Biological Science, Kunsan National University, Gunsan 54150, Republic of Korea.
Center for Convergent Agrobioengineering, Kunsan National University, Gunsan 54150, Republic of Korea.
Pathogens. 2025 Jun 20;14(7):610. doi: 10.3390/pathogens14070610.
is a globally distributed fungal pathogen responsible for significant agricultural losses across a wide range of crops. This study aimed to develop polymorphic simple sequence repeat (SSR) markers by whole-genome resequencing of three Korean isolates and a public reference genome. A total of 16,885 SSR motifs were identified, of which 368 overlapped with polymorphic insertion-deletion (InDel) sites across the four genomes. From these, 12 SSR markers were selected based on polymorphism information content and amplification quality. Validation across the 28 isolates in Korea revealed high levels of genotypic diversity, suggesting that each isolate is a unique haplotype, although is homothallic and clonally propagated. This multi-genome approach provides robust resources for genotyping, molecular diagnostics, and epidemiological surveillance of .
是一种全球分布的真菌病原体，会给多种作物造成重大农业损失。本研究旨在通过对三株韩国分离株和一个公共参考基因组进行全基因组重测序来开发多态性简单序列重复（SSR）标记。共鉴定出16885个SSR基序，其中368个与四个基因组中的多态性插入缺失（InDel）位点重叠。从中，根据多态性信息含量和扩增质量选择了12个SSR标记。对韩国的28个分离株进行验证，结果显示出高水平的基因型多样性，这表明尽管是同宗配合且通过克隆繁殖，但每个分离株都是独特的单倍型。这种多基因组方法为该菌的基因分型、分子诊断和流行病学监测提供了强大的资源。
