Sahlan Muhammad, Rosarina Desy, Ratna Suminar Hasna Farida, Diatama Pohan Yoga, Hidayatullah Ibnu Maulana, Narawangsa Dimas Rafi, Putri Dwini Normayulisa, Sari Eka, Perdani Meka Saima, Wibowo Yudha Gusti, Hermansyah Heri
Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, West Java, Indonesia.
Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, West Java, Indonesia.
Pharmaceutics. 2025 Jun 24;17(7):818. doi: 10.3390/pharmaceutics17070818.
: Solvent-intensive methods are traditionally required to extract curcumin, a potent bioactive compound from , raising environmental and safety concerns. : This study introduces an efficient and scalable extraction approach using microwave-ultrasound-assisted extraction (MUAE) combined with a natural deep eutectic solvent (NADES) composed of choline chloride and lactic acid. Process parameters, including solvent water content (20-30% /) and solid loading (4-8% /), were optimized using response surface methodology (RSM) to enhance curcumin yield. Under optimal conditions (20% water content and 8% solid loading), the MUAE method achieved a curcumin content of 40.72 ± 1.21 mg/g, representing a 14.36% improvement over conventional ultrasound-assisted extraction (UAE), while reducing solvent usage by 50%. The quadratic model demonstrated excellent predictive capability, with an R value of 0.98. In addition, anti-solvent precipitation using water increased curcuminoid purity from 0.31% to 20.54%, with a recovery rate of 21.49%. Mechanistic analysis revealed that microwave-induced cell disruption, ultrasound cavitation, and the modulation of NADES viscosity contributed synergistically to the enhanced extraction performance. This study is the first to combine MUAE with NADES for optimized curcumin extraction, delivering both high yield and reduced solvent consumption. The proposed method offers a sustainable and industrially relevant alternative for curcumin recovery in the food, nutraceutical, and pharmaceutical sectors.
传统上需要采用溶剂密集型方法从姜黄中提取姜黄素，这是一种具有强大生物活性的化合物，引发了环境和安全方面的担忧。本研究引入了一种高效且可扩展的提取方法，即微波 - 超声辅助提取（MUAE），并结合由氯化胆碱和乳酸组成的天然深共熔溶剂（NADES）。使用响应面法（RSM）对包括溶剂含水量（20 - 30% /）和固体负载量（4 - 8% /）在内的工艺参数进行了优化，以提高姜黄素产量。在最佳条件（含水量20%和固体负载量8%）下，MUAE方法获得的姜黄素含量为40.72±1.21 mg/g，比传统超声辅助提取（UAE）提高了14.36%，同时溶剂用量减少了50%。二次模型显示出出色的预测能力，R值为0.98。此外，用水进行反溶剂沉淀将姜黄素类化合物的纯度从0.31%提高到了20.54%，回收率为21.49%。机理分析表明，微波诱导的细胞破裂、超声空化以及NADES粘度的调节协同作用，提高了提取性能。本研究首次将MUAE与NADES结合用于优化姜黄素提取，实现了高产率和减少溶剂消耗。所提出的方法为食品、营养保健品和制药行业中姜黄素的回收提供了一种可持续且与工业相关的替代方案。
