Haloimpatiens sporogenes sp. nov. and Haloimpatiens myeolchijeotgali sp. nov., anaerobic bacteria isolated from Myeolchi-jeot, a traditional Korean fermented anchovy.

Two rod-shaped, Gram-positive, spore-forming, motile, and strictly anaerobic bacteria, FM7315T and FM7330T were isolated from Myeolchi-jeot, a traditional Korean fermented anchovy. Phylogenetic and phylogenomic analyses based on the 16S rRNA gene and genome sequences revealed that strains FM7315T and FM7330T represent novel species within the genus Haloimpatiens. The genome sizes of strains FM7315T and FM7330T were 3,052,517 bp and 4,194,114 bp, respectively, with G + C contents of 29.7 mol% and 28.0 mol%, respectively. Strain FM7315T exhibited growth at 20-37°C, 0-2% NaCl, and pH range of 5.0-8.0, whereas strain FM7330T grew at 25-45°C, 0-4% NaCl, and pH range of 5.0-9.0. Strain FM7315T contains C14:0, C16:0, C18:1 ω9c, Summed Feature 3 (C16:1 ω7c/C16:1 ω6c), and Summed Feature 8 (C18:1 ω7c/C18:1 ω6c) as major fatty acids, along with diphosphatidylglycerol, phosphatidylglycerol, glycolipid, two aminophospholipids, and five unidentified lipids. Strain FM7330T contains C16:0, C17:1 ω8c, and C18:1 ω9c as major fatty acids, along with diphosphatidylglycerol, two phosphatidylglycerols, four aminophospholipids, and six unidentified lipids. Based on their phenotypic, chemotaxonomic, and molecular characteristics, strains FM7315T and FM7330T represent two novel species of the genus Haloimpatiens, for which the names Haloimpatiens sporogenes sp. nov. (FM7315T = KCTC 25939T = JCM 37574T) and Haloimpatiens myeolchijeotgali sp. nov. (FM7330T = KCTC 25938T = JCM 37575T) have been proposed.