Hu Yingying, Liu Weitao, Zhang Yutao, Hu Xuelong
Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Shandong University of Aeronautics, Binzhou, China.
College of Energy and Mining Engineering, Shandong University of Science and Technology, Qingdao, China.
Front Microbiol. 2025 Aug 7;16:1653557. doi: 10.3389/fmicb.2025.1653557. eCollection 2025.
This study investigated the impact of key factors on spore germination of , a self-healing bacterium for concrete, and elucidated its impermeability mechanism to provide theoretical and practical guidance for advanced self-healing concrete development. Controlled experiments determined optimal germination conditions: 2 g/L microcapsule concentration, pH 8, and 1 g/L inosine, yielding peak germination efficiency that highlights parameter synergies. Thermal stimulation for 3 minutes effectively triggered germination, presenting a practical activation approach. MIP and SEM analyses were employed to characterize concrete microstructure. Results showed the alkaline concrete matrix facilitated , while Ca had no inhibitory effect, enabling calcium-based additives in formulations. -containing mortar enhanced cement hydration stability; MIP revealed self-healing concrete had an infiltration fractal cone number of 2.832 and trunk fractal dimension of 2.306, similar to conventional materials, indicating no increased structural complexity. Environmental erosion primarily affects 300-10,000 nm pores, pinpointing durability targets. SEM and MIP analyses confirmed -induced vaterite and aragonite calcium carbonate crystals integrated with tobermorite, reducing porosity and enhancing mechanical strength. These findings indicate the bacterium's potential in self-healing systems, though future research should address complex physicochemical influences and bacterial gradient domestication to improve environmental adaptability.
本研究调查了关键因素对一种用于混凝土的自愈合细菌芽孢萌发的影响，并阐明了其抗渗机制，为先进自愈合混凝土的开发提供理论和实践指导。对照实验确定了最佳萌发条件：微胶囊浓度2 g/L、pH值8和肌苷1 g/L，产生的峰值萌发效率突出了参数协同作用。3分钟的热刺激有效地触发了萌发，提出了一种实际的激活方法。采用汞侵入孔隙率测定法（MIP）和扫描电子显微镜（SEM）分析来表征混凝土微观结构。结果表明，碱性混凝土基体促进了（此处原文缺失细菌名称），而钙没有抑制作用，使得配方中可以使用钙基添加剂。含（此处原文缺失细菌名称）的砂浆提高了水泥水化稳定性；MIP显示自愈合混凝土的渗透分形锥数为2.832，主干分形维数为2.306，与传统材料相似，表明结构复杂性没有增加。环境侵蚀主要影响300 - 10000 nm的孔隙，确定了耐久性目标。SEM和MIP分析证实（此处原文缺失细菌名称）诱导的球霰石和文石碳酸钙晶体与雪硅钙石结合，降低了孔隙率并提高了机械强度。这些发现表明该细菌在自愈合系统中的潜力，不过未来的研究应解决复杂的物理化学影响和细菌梯度驯化问题，以提高环境适应性。
