Shivakumar Chandana B, Raju Nithya Rani, Ramu Pruthvi G, Vishwanath Prashant M, Silina Ekaterina, Stupin Victor, Achar Raghu Ram
Division of Biochemistry, School of Life Sciences, Mysuru, JSS Academy of Higher Education and Research, Mysuru 570015, Karnataka, India.
Department of Biochemistry, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru 570015, Karnataka, India.
Pharmaceutics. 2025 Jul 23;17(8):953. doi: 10.3390/pharmaceutics17080953.
: A nanostructured membrane of polycaprolactone (a synthetic polymer) was synthesized using an electrospinning technique aiming to enhance its hydrophilicity and rate of degradation by surface modification via aminolysis. Since polycaprolactone nanofibrous films are naturally hydrophobic and with slow degradation, which restricts their use in biological systems, amino groups were added to the fiber surface using the aminolysis technique, greatly increasing the wettability of the membranes. : Polycaprolactone nanofibrous membranes were synthesized via the electrospinning technique and surface modification by aminolysis. Trypsin, pepsin, and pancreatin were conjugated onto the aminolyzed PNF surface to further strengthen biocompatibility by enhancing the hydrophilicity, porosity, and biodegradation rate. SEM, FTIR, EDX, and liquid displacement method were performed to investigate proteolytic efficiency and morphological and physical characteristics such as hydrophilicity, porosity, and degradation rates. : Enzyme activity tests, which showed a zone of clearance, validated the successful enzyme conjugation and stability over a wide range of pH and temperatures. Scanning electron microscopy (SEM) confirms the smooth morphology of nanofibers with diameters ranging from 150 to 950 nm. Fourier transform infrared spectroscopy (FTIR) revealed the presence of O-H, C-O, C=O, C-N, C-H, and O-H functional groups. Energy-dispersive X-ray (EDX) elemental analysis indicates the presence of carbon, oxygen, and nitrogen atoms owing to the presence of peptide and amide bonds. The liquid displacement technique and contact angle proved that Pepsin-PNFs possess notably increased porosity (88.50% ± 0.31%) and hydrophilicity (57.6° ± 2.3 (L), 57.9° ± 2.5 (R)), respectively. Pancreatin-PNFs demonstrated enhanced enzyme activity and degradation rate on day 28 (34.61%). : These enzyme-conjugated PNFs thus show improvements in physicochemical properties, making them ideal candidates for various biomedical applications. Future studies must aim for optimization of enzyme conjugation and in vitro and in vivo performance to investigate the versatility of these scaffolds.
采用静电纺丝技术合成了聚己内酯（一种合成聚合物）的纳米结构膜，旨在通过氨解表面改性提高其亲水性和降解速率。由于聚己内酯纳米纤维膜天然疏水且降解缓慢，限制了它们在生物系统中的应用，因此使用氨解技术将氨基添加到纤维表面，大大提高了膜的润湿性。：通过静电纺丝技术合成聚己内酯纳米纤维膜并进行氨解表面改性。将胰蛋白酶、胃蛋白酶和胰酶偶联到氨解的PNF表面，通过提高亲水性、孔隙率和生物降解速率进一步增强生物相容性。进行扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、能量色散X射线（EDX）和液体置换法，以研究蛋白水解效率以及亲水性、孔隙率和降解速率等形态和物理特性。：酶活性测试显示有清晰区域，验证了酶偶联成功以及在广泛的pH和温度范围内的稳定性。扫描电子显微镜（SEM）证实了直径在150至950nm范围内的纳米纤维的光滑形态。傅里叶变换红外光谱（FTIR）揭示了O-H、C-O、C=O、C-N、C-H和O-H官能团的存在。能量色散X射线（EDX）元素分析表明，由于肽键和酰胺键的存在，存在碳、氧和氮原子。液体置换技术和接触角证明，胃蛋白酶-PNFs的孔隙率（88.50%±0.31%）和亲水性（左57.6°±2.3，右57.9°±2.5）分别显著增加。胰酶-PNFs在第28天表现出增强的酶活性和降解率（34.61%）。：这些酶偶联的PNF因此在物理化学性质上有所改善，使其成为各种生物医学应用的理想候选者。未来的研究必须致力于优化酶偶联以及体外和体内性能，以研究这些支架的多功能性。
