Sarmiento-Rubiano Luz A, Goenaga Leidys, Suarez-Marenco Marianella, Gutierrez-Castañeda Clara, Sarmiento Carmen M, Becerra Enríquez Jimmy
Grupo de Investigación Alimentación y Comportamiento Humano, Universidad Metropolitana, Barranquilla, Colombia.
Grupo de Investigación Gestión Ecológica y Agroindustrial, Programa de Microbiología, Universidad Libre, Barranquilla, Colombia.
Rev Peru Med Exp Salud Publica. 2025 Aug 25;42(2):184-189. doi: 10.17843/rpmesp.2025.422.14321.
Motivation for the study. There is little information on the microbiota found in the colostrum of women living in the Colombian Caribbean. Identifying and isolating microorganisms in this fluid is of interest to both medicine and industry. Main findings. The dominant bacterial genera found in colostrum were Staphylococcus, Lactobacillus, and Enterococcus. Microorganisms with probiotic potential were isolated and should be studied in greater detail. Public health implications. Understanding the microbiota found in colostrum allows us to understand its contribution to infant health and develop strategies to enhance the beneficial impact of these microorganisms on their development.
With the aim of isolating and identifying bacteria present in the colostrum of women in the city of Barranquilla, located in the Colombian Caribbean, we carried out a descriptive study on 55 colostrum samples, which were cultured on M17, MRS, and TOS agar and incubated under aerobic and anaerobic conditions. A total of 350 microorganisms were isolated, of which 296 were identified at the genus level by PCR-RFLP with the enzymes HaeIII and RsaI. The online program kodebio.shinyapps.io/RFLP-inator was used for the in-silico identification of the isolates. Seven hypothetical bacterial genera were identified: Staphylococcus, Lactobacillus, Enterococcus, Bifidobacterium, Corynobacterium, Streptococcus, and Leuconostoc, with Staphylococcus, Lactobacillus, and Enterococcus being the most dominant, representing 61.1% of the identified microorganisms. Our results are a starting point for understanding the microbial composition of human colostrum, considering the particular context and environmental conditions of the Colombian Caribbean.
Motivation for the study. There is little information on the microbiota found in the colostrum of women living in the Colombian Caribbean. Identifying and isolating microorganisms in this fluid is of interest to both medicine and industry. Main findings. The dominant bacterial genera found in colostrum were Staphylococcus, Lactobacillus, and Enterococcus. Microorganisms with probiotic potential were isolated and should be studied in greater detail. Public health implications. Understanding the microbiota found in colostrum allows us to understand its contribution to infant health and develop strategies to enhance the beneficial impact of these microorganisms on their development.
研究动机。关于生活在哥伦比亚加勒比地区的女性初乳中的微生物群信息极少。鉴定和分离这种液体中的微生物对医学和工业都具有重要意义。主要发现。初乳中发现的主要细菌属为葡萄球菌、乳杆菌和肠球菌。具有益生菌潜力的微生物已被分离出来，应进行更详细的研究。对公共卫生的影响。了解初乳中的微生物群有助于我们了解其对婴儿健康的贡献，并制定策略以增强这些微生物对婴儿发育的有益影响。
为了分离和鉴定位于哥伦比亚加勒比地区的巴兰基亚市女性初乳中存在的细菌，我们对55份初乳样本进行了描述性研究，这些样本在M17、MRS和TOS琼脂上培养，并在需氧和厌氧条件下孵育。总共分离出350种微生物，其中296种通过使用HaeIII和RsaI酶的PCR-RFLP在属水平上进行了鉴定。使用在线程序kodebio.shinyapps.io/RFLP-inator对分离株进行电子鉴定。鉴定出七个假定的细菌属：葡萄球菌、乳杆菌、肠球菌、双歧杆菌、棒状杆菌、链球菌和明串珠菌，其中葡萄球菌、乳杆菌和肠球菌最为占主导地位，占已鉴定微生物的61.1%。考虑到哥伦比亚加勒比地区的特殊背景和环境条件，我们的研究结果是了解人类初乳微生物组成的一个起点。
研究动机。关于生活在哥伦比亚加勒比地区的女性初乳中的微生物群信息极少。鉴定和分离这种液体中的微生物对医学和工业都具有重要意义。主要发现。初乳中发现的主要细菌属为葡萄球菌、乳杆菌和肠球菌。具有益生菌潜力的微生物已被分离出来，应进行更详细的研究。对公共卫生的影响。了解初乳中的微生物群有助于我们了解其对婴儿健康的贡献，并制定策略以增强这些微生物对婴儿发育的有益影响。
