Gao Shipeng, Xu Xinhao, Zheng Xueyun, Zhang Yang, Zhang Xinai
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Key Laboratory of Fermentation Engineering (Ministry of Education), School of Biological Engineering and Food, Hubei University of Technology, Wuhan 430068, China.
Foods. 2025 Sep 17;14(18):3229. doi: 10.3390/foods14183229.
The growing global demand for rapid, sensitive, and cost-effective food safety monitoring has driven the development of nanozyme-based biosensors as alternatives to natural enzyme-based methods. Among various nanozymes, bimetallic gold-platinum (AuPt) nanozymes show superior catalytic performance compared to monometallic and other Au-based bimetallic hybrids. This is due to their synergistic colorimetric, catalytic, geometric, and ensemble properties. This review systematically evaluates AuPt nanozymes in food safety applications, focusing on their synthesis, structural design, and practical uses. Various structural types are highlighted, including plain, magnetic, porous nanomaterial-labeled, and flexible nanomaterial-loaded AuPt hybrids. Key synthesis methods such as seed-mediated growth and one-pot procedures with different reducing agents are summarized. Detection modes covered include colorimetric, electrochemical, and multimodal sensing, demonstrating efficient detection of important food contaminants. Key innovations include core-shell designs for enhanced catalytic activity, new synthesis strategies for improved structural control, and combined detection modes to increase reliability and reduce false positives. Challenges and future opportunities are discussed, such as standardizing synthesis protocols, scaling up production, and integration with advanced sensing platforms. This review aims to accelerate the translation of AuPt nanozyme technology into practical food safety monitoring solutions that improve food security and public health.
全球对快速、灵敏且经济高效的食品安全监测需求不断增长，推动了基于纳米酶的生物传感器的发展，以替代基于天然酶的方法。在各种纳米酶中，双金属金铂（AuPt）纳米酶与单金属及其他基于金的双金属杂化物相比，具有卓越的催化性能。这归因于它们协同的比色、催化、几何和整体性质。本综述系统评估了AuPt纳米酶在食品安全应用中的情况，重点关注其合成、结构设计和实际用途。突出了各种结构类型，包括普通型、磁性型、多孔纳米材料标记型和柔性纳米材料负载型AuPt杂化物。总结了关键的合成方法，如种子介导生长法和使用不同还原剂的一锅法。涵盖的检测模式包括比色法、电化学法和多模态传感，展示了对重要食品污染物的高效检测。关键创新包括用于增强催化活性的核壳设计、用于改善结构控制的新合成策略以及组合检测模式以提高可靠性并减少假阳性。讨论了挑战和未来机遇，如合成方案的标准化、扩大生产规模以及与先进传感平台的集成。本综述旨在加速将AuPt纳米酶技术转化为实用的食品安全监测解决方案，以改善食品安全和公众健康。
