正常和感染白血病病毒的鸡胚细胞中的脱氧核糖核酸聚合酶活性
Deoxyribonucleic acid polymerase activities in normal and leukovirus-infected chicken embryo cells.
作者信息
Weissbach A, Bolden A, Muller R, Hanafusa H, Hanafusa T
出版信息
J Virol. 1972 Sep;10(3):321-7. doi: 10.1128/JVI.10.3.321-327.1972.
Abstract
Chicken embryo cells normally contain, in addition to deoxyribonucleic acid (DNA)-dependent DNA (D-DNA) polymerases, a novel "R-DNA-polymerase" which specifically copies polyriboadenylic acid strands. This R-DNA polymerase cannot copy natural ribonucleic acid or polyribocytidylic acid strands to a significant extent. Infection of cells with the leukovirus RAV-2 leads to the intracellular formation of large amounts of the viral RNA-dependent DNA polymerase whose properties differ from the cell R-DNA polymerase. Chicken cells transformed by a Rous sarcoma virus mutant which produce noninfectious alpha-type Rous sarcoma virus (f), a leukovirus known to be deficient in the viral RNA-dependent DNA polymerase, do not contain detectable viral RNA-dependent DNA polymerase, whereas the cellular R-DNA polymerase is found in normal amounts. There seems to be no relationship between the cellular R-DNA polymerase and the RNA-dependent DNA polymerase of the avian leukoviruses.
摘要
鸡胚细胞除了含有依赖脱氧核糖核酸（DNA）的DNA（D-DNA）聚合酶外，通常还含有一种新型的“R-DNA聚合酶”，它能特异性地复制聚核糖腺苷酸链。这种R-DNA聚合酶在很大程度上不能复制天然核糖核酸或聚核糖胞苷酸链。用白血病病毒RAV-2感染细胞会导致细胞内大量形成病毒RNA依赖性DNA聚合酶，其特性与细胞R-DNA聚合酶不同。由劳斯肉瘤病毒突变体转化的鸡细胞产生无感染性的α型劳斯肉瘤病毒（f），已知该白血病病毒缺乏病毒RNA依赖性DNA聚合酶，这些细胞中检测不到病毒RNA依赖性DNA聚合酶，而细胞R-DNA聚合酶的含量正常。禽白血病病毒的细胞R-DNA聚合酶与RNA依赖性DNA聚合酶之间似乎没有关系。
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The defectiveness of Rous sarcoma virus.劳氏肉瘤病毒的缺陷性
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Existence and expression of tumor virus genes in chick embryo cells.肿瘤病毒基因在鸡胚细胞中的存在与表达。
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