Lennon V, Mackay I R
Clin Exp Immunol. 1972 Aug;11(4):595-603.
A sensitive gel filtration redioimmunoassay was used to test for antibodies to the basic protein of myelin, the antigen of experimental autoimmune encephalomyelitis, in serum and cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) and other diseases. Sera and CSF from patients with MS gave results similar to those for controls including subjects with various neurological diseases. Binding of I-basic protein by seven-fold concentrated CSF was shown to be due to α-globulin. Free basic protein, as a possible auto-immunogen, was sought in the serum and seven-fold concentrated CSF of patients with MS and controls by competitive inhibition in the radioimmunoassay, but none was demonstrable. If an immunological mechanism is to be invoked in the initiation of destruction of the basic protein of myelin in MS, then either pathogenic `antibody' must be absorbed to the target antigen in the central nervous system or the initiating events must be mediated entirely by sensitized lymphocytes.
采用灵敏的凝胶过滤放射免疫分析法，检测多发性硬化症（MS）患者及其他疾病患者血清和脑脊液（CSF）中针对髓鞘碱性蛋白（实验性自身免疫性脑脊髓炎的抗原）的抗体。MS患者的血清和脑脊液检测结果与对照组（包括患有各种神经疾病的受试者）相似。七倍浓缩脑脊液与I-碱性蛋白的结合表明是由于α-球蛋白所致。通过放射免疫分析中的竞争性抑制，在MS患者和对照组的血清及七倍浓缩脑脊液中寻找作为可能自身免疫原的游离碱性蛋白，但均未检测到。如果要在MS中髓鞘碱性蛋白破坏起始过程中援引免疫机制，那么要么致病“抗体”必须被中枢神经系统中的靶抗原吸收，要么起始事件必须完全由致敏淋巴细胞介导。
