对人类和常见实验动物血浆中鲎试剂检测呈阳性的调查。
Survey for positive Limulus amoebocyte lysate test in plasma from humans and common research animals.
作者信息
DuBose D, Lemaire M, Brown J, Wolfe D, Hamlet M
出版信息
J Clin Microbiol. 1978 Feb;7(2):139-41. doi: 10.1128/jcm.7.2.139-141.1978.
Abstract
A survey for positive Limulus amoebocyte lysate tests was conducted on apparently healthy humans, mongrel dogs, rats, mice, rabbits, and squirrel monkeys. Only mongrel dog (45.8%) and human (32.8%) plasma samples gave positive tests. In dogs, a significant correlation between positive Limulus amoebocyte lysate tests and the presence of intestinal parasites was found. Positives found in human plasma samples were thought to be due to the presence of background levels of endotoxin or some possible mimicker substance found in the plasma after chloroform extraction. It was concluded that there was a need to distinguish between these positive Limulus tests and those which represent significant endotoxemia.
摘要
对看似健康的人类、杂种狗、大鼠、小鼠、兔子和松鼠猴进行了鲎试剂阳性检测调查。只有杂种狗（45.8%）和人类（32.8%）的血浆样本检测呈阳性。在狗身上，发现鲎试剂阳性检测结果与肠道寄生虫的存在之间存在显著相关性。人类血浆样本中发现的阳性结果被认为是由于血浆中存在背景水平的内毒素或氯仿提取后在血浆中发现的一些可能的模拟物质。得出的结论是，有必要区分这些鲎试剂阳性检测结果和那些代表显著内毒素血症的结果。
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本文引用的文献
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N Engl J Med. 1970 Dec 10;283(24):1313-6. doi: 10.1056/NEJM197012102832404.
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The Limulus coagulation test for endotoxin. A comparison with other assay methods.鲎试剂检测内毒素。与其他检测方法的比较。
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