Smiley J D, Sachs C, Ziff M
J Clin Invest. 1968 Mar;47(3):624-32. doi: 10.1172/JCI105758.
A technique for the in vitro culture of rheumatoid synovial tissue with (14)C-amino acids and isolation and quantitation of the newly synthesized immunoglobulins has been developed. This technique has been used to compare immunoglobulin synthesis of 12 rheumatoid synovia with that of synovia from nonarthritic patients and with that of normal human lymph nodes and spleen. In addition, the spleen of a patient with Felty's syndrome has also been examined. Immunoglobulin synthesis in rheumatoid synovia has been shown to be quantitatively and qualitatively similar to that of normal human spleen and lymph nodes although somewhat less active than the Felty's syndrome spleen examined. 79% of the immunoglobulin produced in rheumatoid synovia was of the IgG type, whereas IgM comprised 10% and IgA, 11% of the total. Less than 10% of the IgM synthesized was found to be rheumatoid factor. A fraction containing approximately 90% of its radioactivity in the form of IgG has been obtained for further studies.
已开发出一种用(^{14}C)氨基酸对类风湿性滑膜组织进行体外培养以及分离和定量新合成免疫球蛋白的技术。该技术已用于比较12个类风湿性滑膜组织与非关节炎患者滑膜组织以及正常人淋巴结和脾脏的免疫球蛋白合成情况。此外，还检查了一名费尔蒂综合征患者的脾脏。类风湿性滑膜组织中的免疫球蛋白合成在数量和质量上已显示与正常人脾脏和淋巴结相似，尽管比所检查的费尔蒂综合征脾脏的活性稍低。类风湿性滑膜组织产生的免疫球蛋白中79%为IgG型，而IgM占总量的10%，IgA占11%。发现合成的IgM中类风湿因子不到10%。已获得一个以IgG形式含有约90%放射性的部分用于进一步研究。
