Dhaliwal H S, Ling N R, Bishop S, Chapel H
Clin Exp Immunol. 1978 Feb;31(2):226-36.
A sensitive rosette test utilizing antibody-coated red cells has been applied to the study of the immunoglobulins on the surface of blood lymphocytes in chronic lymphocytic leukaemia (CLL). Contrary to other reports, IgG has been found to be a common surface membrane immunoglobulin (SmIg) on CLL cells. The reasons for this variation are discussed. Evidence is presented to show that (a) the anti-IgG-coated erythrocytes really are detecting IgG and not a cross-reacting substance, (b) the IgG is intrinsic to the cell and not cytophilically bound and (c) Fc-binding and other artefacts have been excluded. Expression of Ig by individual cells was studied by using mixtures of fluorescein- and rhodamine-labelled red cells coated with various anti-Ig. Fifty-one cases of untreated CLL were tested and the lymphocytes of thirty-eight of these cases bore Ig of a single light chain type. These cases could be classified on the basis of their lymphocyte SmIg as follows: eighteen expressing M and D and G, eleven expressing D and G but not M, six expressing M and D but not G, and three expressing G alone.
一种利用抗体包被红细胞的敏感玫瑰花结试验已应用于慢性淋巴细胞白血病（CLL）患者血液淋巴细胞表面免疫球蛋白的研究。与其他报道相反，已发现IgG是CLL细胞上常见的表面膜免疫球蛋白（SmIg）。文中讨论了这种差异的原因。有证据表明：（a）抗IgG包被的红细胞确实检测到的是IgG而非交叉反应物质；（b）IgG是细胞固有的，而非嗜细胞性结合；（c）已排除Fc结合及其他人为因素。通过使用涂有各种抗Ig的荧光素和罗丹明标记红细胞的混合物，研究了单个细胞的Ig表达。检测了51例未经治疗的CLL患者，其中38例患者的淋巴细胞带有单一轻链型的Ig。这些病例可根据其淋巴细胞SmIg分类如下：18例表达M、D和G；11例表达D和G但不表达M；6例表达M和D但不表达G；3例仅表达G。
