Eichmann K, Greenblatt J
J Exp Med. 1971 Mar 1;133(3):424-41. doi: 10.1084/jem.133.3.424.
After repeated intravenous injections with Group C streptococcal vaccine, most rabbit antisera were shown to contain one or more IgG antibody components, as revealed by microzone electrophoresis. A procedure for the fractionation of multiple IgG antibody components from such streptococcal antisera is described. Separation is achieved on the basis of differences in relative binding affinities of the antibody components to immunoabsorbent columns. The evidence suggests that the electrophoretic mobility, and thus the net charge of an antibody, bears a reciprocal relationship to its binding affinity for the streptococcal Group C antigens. Furthermore, the relative binding affinity affords another means to assess the functional homogeneity of streptococcal antibodies. A possible relationship between light chain variable-region subclasses and binding affinities of streptococcal antibodies is discussed.
在用C组链球菌疫苗反复静脉注射后，通过微区电泳显示，大多数兔抗血清含有一种或多种IgG抗体成分。本文描述了一种从这种链球菌抗血清中分离多种IgG抗体成分的方法。分离是基于抗体成分与免疫吸附柱的相对结合亲和力的差异来实现的。有证据表明，抗体的电泳迁移率以及净电荷与其对C组链球菌抗原的结合亲和力呈反比关系。此外，相对结合亲和力提供了另一种评估链球菌抗体功能同质性的方法。本文还讨论了轻链可变区亚类与链球菌抗体结合亲和力之间的可能关系。
