Sugai S, Pillarisetty R, Talal N
J Exp Med. 1973 Oct 1;138(4):989-1002. doi: 10.1084/jem.138.4.989.
Serum monoclonal macroglobulins were detected in over 30% of NZB/NZW F(1) mice greater than 11 mo of age. The monoclonal nature of the IgM was shown by restricted electrophoretic mobility, characteristic appearance on immunoelectrophoresis, restriction to a single light chain type, and ability to induce anti-idiotypic antisera. The monoclonal macroglobulins were separated from antibodies to DNA and RNA that migrated in the 7S region of sucrose gradients. Enlarged lymph nodes were often present in mice with monoclonal IgM, and a transplantable IgM-producing lymphoid tumor was established from the spleen of one animal.
在超过30%的11月龄以上的NZB/NZW F(1)小鼠中检测到血清单克隆巨球蛋白。IgM的单克隆性质通过受限的电泳迁移率、免疫电泳上的特征性外观、限于单一轻链类型以及诱导抗独特型抗血清的能力得以证明。单克隆巨球蛋白与在蔗糖梯度7S区域迁移的针对DNA和RNA的抗体分离。单克隆IgM的小鼠中常出现肿大的淋巴结，并且从一只动物的脾脏建立了可移植的产生IgM的淋巴瘤。
