Knoop F C
Infect Immun. 1979 Jan;23(1):31-3. doi: 10.1128/iai.23.1.31-33.1979.
Experimental enterocolitis was induced in guinea pigs by intraperitoneal injection of clindamycin. Specimens of feces were collected daily in phosphate-buffered saline (pH 7.0) and pooled every second day. The pooled samples were centrifuged to remove solids, and the supernatant was sterilized by membrane filtration. The sterile fecal supernatants were then dialyzed for 48 h against two 15-liter changes of phosphate-buffered saline and subsequently tested for toxicity in cultured monolayers of mouse adrenal cells. A filterable toxin(s) was found in the fecal supernatants on days 2, 4, and 6 postchallenge and not in pretreatment samples. The toxin(s) caused enterocolitis when administered orogastrically to healthy animals and altered the morphology of cultured mouse adrenal cells. The alteration of adrenal cell morphology was neutralized by specific antitoxin to Clostridium histolyticum.
通过腹腔注射克林霉素在豚鼠中诱发实验性小肠结肠炎。每天在磷酸盐缓冲盐水（pH 7.0）中收集粪便样本，每两天合并一次。将合并的样本离心以去除固体，上清液通过膜过滤进行灭菌。然后将无菌粪便上清液在磷酸盐缓冲盐水中透析48小时，换液两次，每次15升，随后在培养的小鼠肾上腺细胞单层中测试其毒性。在攻击后第2、4和6天在粪便上清液中发现了一种可过滤毒素，而在预处理样本中未发现。当经口胃途径给予健康动物时，该毒素会导致小肠结肠炎，并改变培养的小鼠肾上腺细胞的形态。肾上腺细胞形态的改变可被溶组织梭状芽孢杆菌的特异性抗毒素中和。
