Butler W F, Wels C M
Biochem J. 1971 May;122(5):647-52. doi: 10.1042/bj1220647.
The glycosaminoglycan contents of samples from cat intervertebral discs were examined by using cetylpyridinium chloride salt elution techniques. The values obtained related to the region of the vertebral column from which they were derived, to the area of the disc, and to water content. In wet tissue there was a significant difference between regions of the vertebral column and between areas of the disc and findings agreed with previous histological reports. The greater part of the glycosaminoglycans present consisted of chondroitin sulphate and dermatan sulphate with smaller amounts of hyaluronic acid; little keratan sulphate was found. The maximum amounts of chondroitin sulphate and dermatan sulphate occurred in the 0.5m-magnesium chloride fractions usually, but moved towards higher molar concentrations in samples derived from some sites, particularly in the lumbar region. Mean values for the water content of the areas of the disc were: nucleus pulposus, 82.4%; inner anulus, 65.6%; outer anulus, 50.5%. The water content was directly related to the amounts of chondroitin sulphate and dermatan sulphate.
采用十六烷基吡啶鎓氯化物盐洗脱技术检测了猫椎间盘样本中的糖胺聚糖含量。所获得的值与样本取自的脊柱区域、椎间盘面积以及含水量有关。在湿组织中，脊柱不同区域之间以及椎间盘不同区域之间存在显著差异，研究结果与之前的组织学报告一致。样本中存在的大部分糖胺聚糖由硫酸软骨素和硫酸皮肤素组成，透明质酸含量较少；仅发现少量硫酸角质素。硫酸软骨素和硫酸皮肤素的最大含量通常出现在0.5M氯化镁组分中，但在取自某些部位（尤其是腰椎区域）的样本中，其最大含量向更高摩尔浓度移动。椎间盘不同区域的含水量平均值分别为：髓核，82.4%；内纤维环，65.6%；外纤维环，50.5%。含水量与硫酸软骨素和硫酸皮肤素的含量直接相关。
