腺病毒SV40杂交病毒的研究。II. 杂交颗粒的缺陷性。
Studies of adenovirus SV40 hybrid viruses. II. Defectiveness of the hybrid particles.
作者信息
Rowe W P, Bau S G
出版信息
J Exp Med. 1965 Nov 1;122(5):955-66. doi: 10.1084/jem.122.5.955.
Abstract
E46(+) virus purified by limiting dilution and plaque isolation in AGMK cells consists of a mixture of hybrid and non-hybrid virus particles. Of 102 plaque isolates obtained in HEK cells, 98 contained no detectable hybrid virus, while 4 contained a small proportion of hybrid virus. Plaque isolates from AGMK cells uniformly induced SV40 neoantigen, but when these plaque isolates were plaqued in HEK cells the progeny were again non-hybrid adenovirus. It thus appears impossible to obtain a pure line of hybrid virus. The proportion of hybrid virus produced in HEK cells fell progressively with decreasing multiplicity of infection, and plaque induction by E46(+) virus in AGMK followed 2-hit kinetics, indicating that the hybrid virus particles are defective and require dual infection with non-hybrid virus for their propagation.
摘要
通过在非洲绿猴肾（AGMK）细胞中进行有限稀释和空斑分离纯化得到的E46（+）病毒由杂交和非杂交病毒颗粒的混合物组成。在人胚肾（HEK）细胞中获得的102个空斑分离株中，98个未检测到杂交病毒，而4个含有少量杂交病毒。来自AGMK细胞的空斑分离株均能诱导SV40新抗原，但当这些空斑分离株在HEK细胞中进行空斑形成时，子代又变为非杂交腺病毒。因此，似乎不可能获得纯系杂交病毒。在HEK细胞中产生的杂交病毒比例随着感染复数的降低而逐渐下降，并且E46（+）病毒在AGMK细胞中的空斑诱导遵循双打击动力学，这表明杂交病毒颗粒存在缺陷，需要与非杂交病毒双重感染才能增殖。
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