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1
Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization.腺病毒SV40杂交病毒的研究。I. 检测系统及杂交的进一步证据。
J Exp Med. 1965 Nov 1;122(5):943-54. doi: 10.1084/jem.122.5.943.
2
Studies of adenovirus SV40 hybrid viruses. II. Defectiveness of the hybrid particles.腺病毒SV40杂交病毒的研究。II. 杂交颗粒的缺陷性。
J Exp Med. 1965 Nov 1;122(5):955-66. doi: 10.1084/jem.122.5.955.
3
A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population.从AD.2-SV40杂交群体中分离出的一种无缺陷（有活性）的腺病毒-SV40杂交体。
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128-35. doi: 10.1073/pnas.63.4.1128.
4
Components of adenovirus-simian virus 40 hybrid viruses.腺病毒-猴病毒40杂交病毒的组成成分。
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1525-9. doi: 10.1073/pnas.68.7.1525.
5
Equilibrium density gradient studies on simian virus 40-yielding variants of the adenovirus type 2-simian virus 40 hybrid population.对腺病毒2型-猴病毒40杂交群体中产生猴病毒40的变体进行的平衡密度梯度研究。
J Virol. 1970 Apr;5(4):421-6. doi: 10.1128/JVI.5.4.421-426.1970.
6
Isolation of two plaque variants from the adenovirus type 2-simian virus 40 hybrid population which differ in their efficiency in yielding simian virus 40.从腺病毒2型-猴病毒40杂交群体中分离出两种蚀斑变体，它们产生猴病毒40的效率不同。
J Virol. 1970 Apr;5(4):413-20. doi: 10.1128/JVI.5.4.413-420.1970.
7
Effect of inhibitors on induction of SV40 tumor antigen by an adenovirus-SV40 hybrid.抑制剂对腺病毒 - SV40杂交体诱导SV40肿瘤抗原的影响。
Arch Gesamte Virusforsch. 1965;17(3):424-35. doi: 10.1007/BF01241197.
8
In vitro transformation by the adenovirus-simian virus 40 hybrid viruses. V. Virus-specific ribonucleic acid in cell lines transformed by the adenovirus 2-simian virus 40 and adenovirus 12-simian virus 40 transcapsidant hybrid viruses.腺病毒-猴病毒40杂交病毒的体外转化。V. 由腺病毒2-猴病毒40和腺病毒12-猴病毒40转壳杂交病毒转化的细胞系中的病毒特异性核糖核酸。
J Virol. 1969 Nov;4(5):704-11. doi: 10.1128/JVI.4.5.704-711.1969.
9
Simian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule.猿猴病毒40在腺病毒7-猿猴病毒40杂交DNA分子中的整合位点。
Proc Natl Acad Sci U S A. 1971 May;68(5):1037-41. doi: 10.1073/pnas.68.5.1037.
10
Replication in simian cells of defective viruses in an SV40-adenovirus "hybrid" population.SV40-腺病毒“杂交”群体中缺陷病毒在猴细胞中的复制。
J Bacteriol. 1966 Jan;91(1):278-84. doi: 10.1128/jb.91.1.278-284.1966.

引用本文的文献

1
Density differences between hybrid and nonhybrid particles in two adenovirus-simian virus 40 hybrid populations.两个腺病毒-猴病毒40杂交群体中杂交颗粒与非杂交颗粒之间的密度差异。
J Virol. 1970 Mar;5(3):353-7. doi: 10.1128/JVI.5.3.353-357.1970.
2
The presence of antibodies in human serum to early (T) adenovirus antigens.人血清中存在针对早期（T）腺病毒抗原的抗体。
Proc Natl Acad Sci U S A. 1967 Mar;57(3):622-9. doi: 10.1073/pnas.57.3.622.
3
Characterization of the tumorlike (T) antigen induced by type 12 adenovirus. II. Physical and chemical properties.12型腺病毒诱导的肿瘤样（T）抗原的特性。II. 物理和化学性质。
J Virol. 1968 Jan;2(1):15-20. doi: 10.1128/JVI.2.1.15-20.1968.
4
Characterization of the tumorlike (T) antigen induced by type 12 adenovirus. I. Purification of the antigen from infected KB cells and a hamster tumor cell line.12型腺病毒诱导的肿瘤样（T）抗原的特性。I. 从感染的KB细胞和仓鼠肿瘤细胞系中纯化该抗原。
J Virol. 1968 Jan;2(1):7-14. doi: 10.1128/JVI.2.1.7-14.1968.
5
Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics.在各种腺病毒制剂中发现的小型DNA病毒的研究：物理、生物学和免疫学特征。
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1467-74. doi: 10.1073/pnas.55.6.1467.
6
Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40.猴病毒40增强猴细胞中人腺病毒感染机制的研究。
J Virol. 1972 Aug;10(2):211-9. doi: 10.1128/JVI.10.2.211-219.1972.
7
Components of adenovirus-simian virus 40 hybrid viruses.腺病毒-猴病毒40杂交病毒的组成成分。
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1525-9. doi: 10.1073/pnas.68.7.1525.
8
In vitro transformation by adenovirus-simiam virus 40 hybrid viruses. IV. Properties of clones isolated from cell lines transformed by adenovirus 2-simiam virus 40 and adenovirus 12-simiam virus 40 transcapsidant hybird viruses.腺病毒-猴病毒40杂交病毒的体外转化。IV. 从由腺病毒2-猴病毒40和腺病毒12-猴病毒40衣壳转导杂交病毒转化的细胞系中分离出的克隆的特性。
J Virol. 1969 Nov;4(5):694-703. doi: 10.1128/JVI.4.5.694-703.1969.
9
Virus-specific nucleic acids in SV40-exposed hamster embryo cell lines: correlation with S and T antigens.暴露于SV40的仓鼠胚胎细胞系中的病毒特异性核酸：与S和T抗原的相关性。
Proc Natl Acad Sci U S A. 1969 Feb;62(2):589-96. doi: 10.1073/pnas.62.2.589.
10
Replication and complementation of human adenoviruses and simian papovavirus at an elevated temperature.人腺病毒和猴乳头瘤空泡病毒在高温下的复制与互补作用
J Virol. 1968 Jul;2(7):670-7. doi: 10.1128/JVI.2.7.670-677.1968.

本文引用的文献

1
Studies on simian virus 40.关于猴病毒40的研究。
J Immunol. 1962 Jun;88:796-806.
2
THE NATURE AND LOCALIZATION OF THE SV 40-INDUCED COMPLEMENT-FIXING ANTIGEN.猴空泡病毒40诱导的补体结合抗原的性质与定位
Proc Natl Acad Sci U S A. 1965 Mar;53(3):684-92. doi: 10.1073/pnas.53.3.684.
3
THE INCORPORATION OF SV40 MATERIAL INTO ADENOVIRUS 7 AS MEASURED BY INTRANUCLEAR SYNTHESIS OF SV40 TUMOR ANTIGEN.通过SV40肿瘤抗原的核内合成来测定SV40物质掺入腺病毒7的情况。
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1348-52. doi: 10.1073/pnas.52.6.1348.
4
EVIDENCE FOR A POSSIBLE GENETIC HYBRID BETWEEN ADENOVIRUS TYPE 7 AND SV40 VIRUSES.7型腺病毒与猿猴空泡病毒40（SV40）之间可能存在基因杂交的证据。
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1340-7. doi: 10.1073/pnas.52.6.1340.
5
INDUCTION BY ADENOVIRUS TYPE 7 OF TUMORS IN HAMSTERS HAVING THE ANTIGENIC CHARACTERISTICS OF SV40 VIRUS.7型腺病毒对具有SV40病毒抗原特性的仓鼠肿瘤的诱导作用
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1333-40. doi: 10.1073/pnas.52.6.1333.
6
TUMOR AND VIRUS ANTIGENS OF SIMIAN VIRUS 40: DIFFERENTIAL INHIBITION OF SYNTHESIS BY CYTOSINE ARABINOSIDE.猴病毒40的肿瘤与病毒抗原：阿糖胞苷对其合成的差异抑制作用
Science. 1965 Feb 5;147(3658):625-7. doi: 10.1126/science.147.3658.625.
7
VIRUS-INDUCED INTRANUCLEAR ANTIGEN IN CELLS TRANSFORMED BY PAPOVAVIRUS SV40.乳头多瘤空泡病毒SV40转化细胞中病毒诱导的核内抗原
Proc Soc Exp Biol Med. 1964 Aug-Sep;116:1131-5. doi: 10.3181/00379727-116-29472.
8
THE PURIFICATION OF SIMIAN VIRUS 40.猿猴病毒40的纯化
Virology. 1964 Nov;24:381-7. doi: 10.1016/0042-6822(64)90175-8.
9
IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES.腺病毒12肿瘤以及被腺病毒转化或感染的细胞的免疫荧光研究
J Exp Med. 1964 Oct 1;120(4):577-88. doi: 10.1084/jem.120.4.577.
10
A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS.SV40肿瘤细胞和转化细胞中存在的一种特异性补体结合抗原。
Proc Natl Acad Sci U S A. 1963 Dec;50(6):1148-56. doi: 10.1073/pnas.50.6.1148.

腺病毒SV40杂交病毒的研究。I. 检测系统及杂交的进一步证据。

Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization.

作者信息

Rowe W P, Baum S G, Pugh E, Hoggan M D

出版信息

J Exp Med. 1965 Nov 1;122(5):943-54. doi: 10.1084/jem.122.5.943.

DOI:10.1084/jem.122.5.943
PMID:4285252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2138118/
Abstract

The titers of adenovirus and SV40 genetic carriers in hybrid preparations (E46(+)) can be quantitated by determining the percentage of cells showing neoantigens stainable with fluorescent antibody (FA) at 21 to 24 hours; both titers can be obtained with a single coverslip. The adenovirus and SV40 antigen-inducing titers so obtained are of the same order of magnitude in stock preparations of E46(+) grown in either African green monkey kidney (AGMK) or human embryonic kidney tissue culture (HEK), and are generally within one log of the infectivity titer. Quantitative studies of 50 degrees C heat inactivation, ultracentrifugation, and equilibrium density gradient centrifugation of E46(+) gave no indication that SV40 neoantigen induction could be dissociated from adenovirus, whereas adenovirus and SV40 virus grown as a mixed infection were readily dissociated by these procedures. Pretreatment of HEK cells with a medium containing 5-fluorouracil desoxyriboside (FUDR) did not affect induction of either adenovirus or SV40 neoantigen or development of cytopathic effects after infection with E46(+), but did prevent formation of Ad. 7 viral antigen.

摘要

杂种制剂（E46(+)）中腺病毒和SV40基因载体的滴度可通过测定在21至24小时时显示可用荧光抗体（FA）染色的新抗原的细胞百分比来定量；两种滴度可通过单个盖玻片获得。在非洲绿猴肾（AGMK）或人胚肾组织培养（HEK）中生长的E46(+)储备制剂中，如此获得的腺病毒和SV40抗原诱导滴度处于相同数量级，并且通常在感染性滴度的一个对数范围内。对E46(+)进行50℃热灭活、超速离心和平衡密度梯度离心的定量研究表明，没有迹象表明SV40新抗原诱导可与腺病毒分离，而作为混合感染生长的腺病毒和SV40病毒很容易通过这些程序分离。用含有5-氟尿嘧啶脱氧核苷（FUDR）的培养基预处理HEK细胞，不影响E46(+)感染后腺病毒或SV40新抗原的诱导或细胞病变效应的发展，但确实可防止Ad. 7病毒抗原的形成。