Lewis A M, Levin M J, Wiese W H, Crumpacker C S, Henry P H
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128-35. doi: 10.1073/pnas.63.4.1128.
A new nondefective hybrid virus has been plaque-isolated from the Ad.2-SV40 hybrid population. This virus replicates efficiently with one-hit kinetics in both human embryonic kidney and African green monkey kidney cells, induces an SV40 specific antigen which is detectable by immunofluorescence and complement-fixation using sera from SV40 tumor-bearing hamsters, and produces SV40-specific RNA detectable by DNA-RNA hybridization. The SV40-specific antigen induced by this virus is heat-stable, sensitive to inhibitors of DNA synthesis, serologically different from SV40 T and viral antigens, and is an unrecognized SV40 antigen.
一种新的无缺陷杂交病毒已从腺病毒2-猴空泡病毒40（Ad.2-SV40）杂交群体中通过空斑分离得到。这种病毒在人胚肾细胞和非洲绿猴肾细胞中均能以单次打击动力学高效复制，诱导出一种SV40特异性抗原，该抗原可通过使用来自携带SV40肿瘤的仓鼠血清进行免疫荧光和补体结合试验检测到，并且能产生可通过DNA-RNA杂交检测到的SV40特异性RNA。由这种病毒诱导产生的SV40特异性抗原具有热稳定性，对DNA合成抑制剂敏感，在血清学上与SV40 T抗原和病毒抗原不同，是一种未被识别的SV40抗原。
