支链脂肪酸掺入黏液病毒。
Incorporation of branched-chain fatty acids into myxoviruses.
作者信息
Blough H A, Tiffany J M
出版信息
Proc Natl Acad Sci U S A. 1969 Jan;62(1):242-7. doi: 10.1073/pnas.62.1.242.
The incorporation of free fatty acids into myxoviruses was shown, using branched-chain fatty acids as molecular markers. The presence of isostearic or phytanic acid was detected by gas-liquid chromatography in the phospholipid fraction of the A(0)/PR8/34 strain of influenza virus. Uptake of free fatty acids into the virus varied from 8 to 11 per cent and was accompanied by a shift in the fatty acid profile. Infected allantoic fluids from eggs treated with branched-chain acids possessed higher hemagglutinin activity when compared to fluids infected under normal conditions. Attempts to detect branched-chain acids in Sendai virus were unsuccessful. Shifts in acyl chain composition persisted after three passages of modified viruses in the absence of branched-chain acids. Force-area curves at an air-water interface revealed the cross-sectional area of branched-chain acids to be greater than their straight-chain homologs. It is suggested that hydrophobic interactions can alter the configuration of envelope proteins. Such changes may have an important role in the selection of fragments of influenza viral genome and can conceivably alter viral genotype.
利用支链脂肪酸作为分子标记，研究了游离脂肪酸掺入黏液病毒的情况。通过气液色谱法在A(0)/PR8/34流感病毒株的磷脂组分中检测到异硬脂酸或植烷酸的存在。游离脂肪酸掺入病毒的比例为8%至11%，并伴随着脂肪酸谱的变化。与正常条件下感染的液体相比，用支链酸处理的鸡蛋感染后的尿囊液具有更高的血凝素活性。在仙台病毒中检测支链酸的尝试未成功。在没有支链酸的情况下，经过三代传代后，修饰病毒的酰基链组成仍发生了变化。气-水界面的力-面积曲线显示，支链酸的横截面积大于其直链同系物。研究表明，疏水相互作用可改变包膜蛋白的构象。这种变化可能在流感病毒基因组片段的选择中起重要作用，并且可以想象会改变病毒基因型。
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