Gulati S C, Axel R, Spiegelman S
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2020-4. doi: 10.1073/pnas.69.8.2020.
An experimental procedure is detailed that permits the detection of 70S RNA-directed DNA synthesis in mouse mammary carcinomas. The DNA synthesized is complementary to the RNA of the mouse mammary tumor virus by molecular hybridization, thus, completing the proof that an RNA-instructed DNA polymerase has been identified. Further, RNA-instructed DNA polymerase and its 70S RNA template are physically associated in a particle that has a density characteristic of oncornaviruses. These experiments now provide the technology required to perform similar examinations of human neoplasias.
详细介绍了一种实验程序，该程序可检测小鼠乳腺癌中70S RNA指导的DNA合成。通过分子杂交，合成的DNA与小鼠乳腺肿瘤病毒的RNA互补，从而完成了已鉴定出RNA指导的DNA聚合酶的证明。此外，RNA指导的DNA聚合酶及其70S RNA模板在具有肿瘤病毒密度特征的颗粒中物理结合。这些实验现在提供了对人类肿瘤进行类似检查所需的技术。
