Darbyshire J H, Hedger R S, Arrowsmith E M
J Hyg (Lond). 1972 Mar;70(1):171-80. doi: 10.1017/s002217240002221x.
A comparison was made between a macrotechnique in tubes and a microtechnique in plastic plates for complement-fixation tests, using strains of three subtypes of the Asia 1 type of foot-and-mouth disease (FMD) virus. The results obtained with these techniques were found to be comparable and delineated the antigenic relationships of the three strains employed. The microtechnique was considered to be both economical with reagents and capable of similar accuracy and reproducibility to that of the standard method in tubes. It was concluded that the antigenic analysis of subtype strains of FMD virus can be conveniently carried out by the use of the microtechnique as described.
采用亚洲1型口蹄疫（FMD）病毒三种亚型毒株，对用于补体结合试验的试管中的宏观技术和塑料板中的微观技术进行了比较。发现用这些技术获得的结果具有可比性，并描绘了所用三种毒株的抗原关系。微观技术被认为既节省试剂，又能达到与标准试管法相似的准确性和可重复性。得出的结论是，口蹄疫病毒亚型毒株的抗原分析可以通过使用所述的微观技术方便地进行。
