RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。V. 感染鸡胚成纤维细胞中的劳斯肉瘤病毒单链RNA-DNA共价杂交体。
RNA-dependent DNA polymerase activity of RNA tumor viruses. V. Rous sarcoma virus single-stranded RNA-DNA covalent hybrids in infected chicken embryo fibroblast cells.
作者信息
Leis J, Schincariol A, Ishizaki R, Hurwitz J
出版信息
J Virol. 1975 Mar;15(3):484-9. doi: 10.1128/JVI.15.3.484-489.1975.
Abstract
RNA-DNA covalent hybrids containing viral RNA have been isolated from nuclear fractions of Rous sarcoma virus-infected chicken embryo fibroblast cells shortly after virus infection. The formation of covalent hybrid structures depends upon a functional reverse transcriptase in vivo, since its appearance in cells is temperature dependent when infected with Rous sarcoma virus mutant LA335, which contains a temperature-sensitive reverse transcriptase.
摘要
在感染劳斯肉瘤病毒后不久，已从感染该病毒的鸡胚成纤维细胞的核组分中分离出含有病毒RNA的RNA-DNA共价杂交体。共价杂交结构的形成取决于体内功能性逆转录酶，因为当用含有温度敏感型逆转录酶的劳斯肉瘤病毒突变体LA335感染细胞时，其在细胞中的出现与温度有关。
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