Rosenkranz H S, Becker Y
Antimicrob Agents Chemother. 1973 Mar;3(3):325-31. doi: 10.1128/AAC.3.3.325.
Hydroxyurea, at a concentration of 5 x 10(-2) M, inhibits the replication of herpes simplex deoxyribonucleic acid (DNA) in the nuclei of infected cells. As a result, the synthesis of infectious virus progeny was prevented. The presence of parental viral DNA genomes in inhibited cells led to the synthesis of the viral structural peptides. The inhibitory effect of hydroxyurea was reversible; after washing the cells free from hydroxyurea, virus progeny appeared after a lag of 3 h. Upon resumption of viral DNA replication, the content of radioactive viral structural peptides gradually increased in parallel with the increase in mature virions. It is concluded that the information for the synthesis of viral structural peptides is transcribed from the parental DNA genomes.
羟基脲在浓度为5×10⁻² M时，可抑制单纯疱疹脱氧核糖核酸（DNA）在受感染细胞核中的复制。结果，感染性病毒子代的合成被阻止。在受抑制的细胞中存在亲代病毒DNA基因组会导致病毒结构肽的合成。羟基脲的抑制作用是可逆的；将细胞洗涤去除羟基脲后，经过3小时的延迟期后出现病毒子代。病毒DNA复制恢复后，放射性病毒结构肽的含量随着成熟病毒粒子数量的增加而逐渐平行增加。得出的结论是，病毒结构肽合成的信息是从亲代DNA基因组转录而来的。
