Hirsch C F, Ensign J C
J Bacteriol. 1976 Apr;126(1):24-30. doi: 10.1128/jb.126.1.24-30.1976.
The lag period preceding germination of Streptomyces viridochromogenes spores during incubation in a defined germination medium was completely eliminated by a gentle heat shock. The rate of germination was not affected. The optimum pH for activation extended from 6.0 to 9.6. The time of heating required for maximum activation was 1 min at 60 C, 2 to 5 min at 55 C, 20 min at 50 C, and 40 to 50 min at 45 C. Activated spores had the same temperature and pH optima and nutritional requirements for germination as unactivated spores. Activated spores deactivated during incubation for 8 h at 25 C and were activated again by a second heat shock. Spores that had been aged for 4 weeks or longer did not germinate in the defined germination medium unless they were first heat activated.
在特定的萌发培养基中培养时，绿色产色链霉菌孢子萌发前的延迟期可通过温和的热激处理完全消除。萌发速率不受影响。激活的最适pH范围为6.0至9.6。在60℃下实现最大激活所需的加热时间为1分钟，55℃下为2至5分钟，50℃下为20分钟，45℃下为40至50分钟。激活后的孢子与未激活的孢子在萌发时具有相同的温度、pH最适值和营养需求。激活后的孢子在25℃下培养8小时会失活，再次通过热激处理可重新激活。储存4周或更长时间的孢子，除非先进行热激活，否则在特定的萌发培养基中不会萌发。
