Eaton D, Ensign J C
J Bacteriol. 1980 Jul;143(1):377-82. doi: 10.1128/jb.143.1.377-382.1980.
Initiation of germination of heat-activated Streptomyces viridochromogenes spore occurs in media containing only calcium ions and organic buffer. The calcium-induced initiation of germination was accompanied by a decrease in absorbance of the spore suspension, an increased rate of endogenous metabolism, the loss of spore carbon, and the loss of heat resistance. Calcium amounts to 0.28% of the dry weight of freshly harvested spores. The amount of calcium remained the same after incubation of spores in water after heat activation. The spore content of calcium doubled after incubation in 0.5 mM CaCl2 for 5 min at 4 degrees C and during calcium-induced germination. Nearly all of the calcim appears to be bound to sites external to the spore membrane, since the chelating agents (ethylenedinitrilo) tetraacetic acid and arsenazo III removed virtually all of the calcium ions. The calcium ions must be present during the entire initiation of germination period. Germination ceases after an (ethylenedinitrilo) tetraacetic acid wash and begins again immediately after addition of calcium ions.
热激活的绿色产色链霉菌孢子在仅含有钙离子和有机缓冲液的培养基中开始萌发。钙离子诱导的萌发起始伴随着孢子悬浮液吸光度的降低、内源性代谢速率的增加、孢子碳的损失以及耐热性的丧失。钙占新鲜收获孢子干重的0.28%。热激活后将孢子在水中孵育，钙含量保持不变。在4℃下于0.5 mM CaCl₂中孵育5分钟以及在钙离子诱导的萌发过程中，孢子中的钙含量翻倍。几乎所有的钙似乎都与孢子膜外部的位点结合，因为螯合剂（乙二胺四乙酸）和偶氮胂III几乎去除了所有的钙离子。在整个萌发起始阶段都必须存在钙离子。用（乙二胺四乙酸）洗涤后萌发停止，添加钙离子后立即再次开始萌发。
