Rouse B T, Babiuk L A
Infect Immun. 1974 Oct;10(4):681-7. doi: 10.1128/iai.10.4.681-687.1974.
Isolated peripheral blood lymphocytes (PBL) from cattle immunized or infected with infectious bovine rhinotracheitis (IBR) virus were cultured in vitro with ultraviolet light-inactivated IBR virus, and the degree of lymphocyte blastogenesis was quantitated by measurement of the uptake of [(3)H]thymidine into acid-insoluble material. Lymphocyte blastogenesis only occurred with PBL from immunized or infected animals. The optimal conditions for lymphocyte blastogenesis were defined. Blastogenesis was specific since cells from animals immunized against IBR failed to react with two other herpesvirus antigens tested, herpes simplex and equine rhinopneumonitis viruses. Blastogenesis could be prevented by reacting IBR antigen with IBR-specific antibody before adding to cultures, but incorporating IBR-specific antibody in the culture medium after adding free antigen failed to inhibit blastogenesis. With intranasally infected animals, lymphocyte blastogenesis was detectable after 5 days, reached peak levels between days 7 and 10, and then declined to low levels by day 19. In contrast, levels of neutralizing antibody were barely detectable on day 7 and reached maximal concentrations on day 19. The lymphocyte blastogenesis assay was emphasized as a convenient and useful in vitro correlate of cell-mediated immunity that should help define the role of cell-mediated immunity in immunity to herpesviruses.
将来自接种或感染传染性牛鼻气管炎（IBR）病毒的牛的外周血淋巴细胞（PBL）与紫外线灭活的IBR病毒在体外进行培养，通过测量[（3）H]胸苷掺入酸不溶性物质的量来定量淋巴细胞的增殖程度。淋巴细胞增殖仅发生在来自免疫或感染动物的PBL中。确定了淋巴细胞增殖的最佳条件。增殖具有特异性，因为来自免疫IBR的动物的细胞不能与另外两种测试的疱疹病毒抗原（单纯疱疹病毒和马鼻肺炎病毒）发生反应。在加入培养物之前，通过使IBR抗原与IBR特异性抗体反应可以阻止增殖，但在加入游离抗原后将IBR特异性抗体加入培养基中并不能抑制增殖。对于鼻内感染的动物，在5天后可检测到淋巴细胞增殖，在第7至10天达到峰值水平，然后在第19天降至低水平。相比之下，中和抗体水平在第7天几乎检测不到，在第19天达到最大浓度。淋巴细胞增殖试验被强调为细胞介导免疫的一种方便且有用的体外相关指标，这应该有助于确定细胞介导免疫在疱疹病毒免疫中的作用。
