起始因子3在以N-乙酰苯丙氨酰-tRNA起始多肽合成中的需求
Requirement of initiation factor 3 in the initiation of polypeptide synthesis with N-acetylphenylalanyl-tRNA.
作者信息
Bernal S D, Blumberg B M, Nakamoto T
出版信息
Proc Natl Acad Sci U S A. 1974 Mar;71(3):774-8. doi: 10.1073/pnas.71.3.774.
Abstract
Initiation factor 3 is required, along with initiation factors 1 and 2, for the incorporation of N-acetylphenylalanine into polypeptides and the formation of N-acetylphenylalanylpuromycin. Initiation factor 3 also strongly stimulates the binding of N-acetylphenylalanyl transfer RNA to isolated 30S ribosomal subunits. Phosphocellulose fractions of initiation factor 3 were found to catalyze N-acetylphenylalanine incorporation differentially with different synthetic messenger RNAs not containing any codons for N-formylmethionine. The results suggest that ribosomes recognize the initiator codon only through the initiator transfer RNA.
摘要
起始因子3与起始因子1和2一起，是将N - 乙酰苯丙氨酸掺入多肽以及形成N - 乙酰苯丙氨酰嘌呤霉素所必需的。起始因子3还强烈刺激N - 乙酰苯丙氨酰转移RNA与分离的30S核糖体亚基的结合。发现起始因子3的磷酸纤维素级分对不同的不含N - 甲酰甲硫氨酸密码子的合成信使RNA催化N - 乙酰苯丙氨酸掺入的情况有所不同。结果表明核糖体仅通过起始转移RNA识别起始密码子。
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本文引用的文献
1
Translation of the genetic message, ii. Effect of initiation factors on the binding of formyl-methionyl-trna to ribosomes.遗传信息的翻译，ii. 起始因子对甲酰甲硫氨酰 - tRNA与核糖体结合的影响。
Proc Natl Acad Sci U S A. 1967 Feb;57(2):387-94. doi: 10.1073/pnas.57.2.387.
2
The initiation of viral protein synthesis in e. Coli extracts.大肠杆菌提取物中病毒蛋白质合成的起始
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1786-93. doi: 10.1073/pnas.56.6.1786.
3
INTERACTION OF RIBOSOMES AND SYNTHETIC POLYRIBONUCLEOTIDES.核糖体与合成多聚核糖核苷酸的相互作用
J Mol Biol. 1963 Oct;7:323-33. doi: 10.1016/s0022-2836(63)80027-3.
4
Fractionation of translation initiation factor B (F3) into cistron-specific species.将翻译起始因子B（F3）分离为顺反子特异性物种。
FEBS Lett. 1970 Aug 17;9(4):213-217. doi: 10.1016/0014-5793(70)80358-1.
5
Interconversions between inactive and active forms of ribosomal subunits.核糖体亚基的无活性形式与活性形式之间的相互转化。
FEBS Lett. 1969 Apr;3(1):85-88. doi: 10.1016/0014-5793(69)80103-1.
6
Studies on the ribosomal initiation factor F(3). Multiplicity of F(3) and DF activities.
FEBS Lett. 1971 Dec 15;19(3):201-206. doi: 10.1016/0014-5793(71)80513-6.
7
Different classes of initiation factors F3 and their dissociation activity.不同类别的起始因子F3及其解离活性。
FEBS Lett. 1971 Dec 15;19(3):193-200. doi: 10.1016/0014-5793(71)80512-4.
8
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