来自兔抗链球菌碳水化合物抗体的同种异型b4轻链的氨基酸序列。
Amino-acid sequence of an allotype b4 light chain from a rabbit antibody to streptococcal carbohydrate.
作者信息
Chen K C, Kindt T J, Krause R M
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1995-8. doi: 10.1073/pnas.71.5.1995.
Abstract
The complete amino-acid sequence was determined for a rabbit-antibody light chain of allotype b4. The light chain was recovered from approximately 20 g of homogeneous antibody to group C streptococcal carbohydrate, which was obtained from an immunized rabbit. Several enzymic digestions were used to obtain the overlapping peptides necessary for the sequence determination. The L chain has 210 amino-acid residues, as determined by sequence analysis, and a molecular mass of 23,060 daltons. This antibody light chain contains seven half-cystine residues characteristic of the (kappa)B light-chain class. The amino-terminal sequence was determined to be Ala-Asp-Ile-Val-Met, indicating that the chain belongs to the V(kappa)I subgroup.
摘要
测定了同种异型b4兔抗体轻链的完整氨基酸序列。该轻链是从约20 g针对C组链球菌碳水化合物的纯一抗体中获得的，此抗体取自一只免疫兔。使用了几种酶促消化方法来获得序列测定所需的重叠肽段。通过序列分析确定，该轻链含有210个氨基酸残基，分子量为23,060道尔顿。此抗体轻链含有7个半胱氨酸残基，这是κB轻链类的特征。其氨基末端序列测定为Ala-Asp-Ile-Val-Met，表明该链属于VκI亚组。
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Proc Natl Acad Sci U S A. 1974 May;71(5):1995-8. doi: 10.1073/pnas.71.5.1995.
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