体外DNA合成研究：从大肠杆菌中纯化具有DNA D活性的DNA C基因产物。
Studies on in vitro DNA synthesis: purification of dna C gene product containing dna D activity from Escherichia coli.
作者信息
Wickner S, Berkower I, Wright M, Hurwitz J
出版信息
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2369-73. doi: 10.1073/pnas.70.8.2369.
Abstract
The conversion of varphiX174 single-stranded DNA to duplex DNA by extracts of E. coli requires products of the E. coli DNA replication genes. By use of this complementation system, the dna C gene product has been purified from wild-type E. coli as well as from a dna C temperature-sensitive mutant. The latter preparations are temperature sensitive when compared to the wild-type gene product. The dna C and dna D gene products copurify, have similar characteristics, are both temperature sensitive in preparations from dna C temperature-sensitive cells, and are both undetectable in preparations from dna D temperature-sensitive cells.
摘要
大肠杆菌提取物将φX174单链DNA转化为双链DNA需要大肠杆菌DNA复制基因的产物。利用这种互补系统，已从野生型大肠杆菌以及dnaC温度敏感突变体中纯化出dnaC基因产物。与野生型基因产物相比，后一种制剂对温度敏感。dnaC和dnaD基因产物共同纯化，具有相似的特性，在来自dnaC温度敏感细胞的制剂中均对温度敏感，而在来自dnaD温度敏感细胞的制剂中均无法检测到。
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本文引用的文献
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Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12.大肠杆菌K-12温度敏感突变体中脱氧核糖核酸的营养复制和转移复制
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Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3. doi: 10.1073/pnas.68.12.3150.
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Escherichia coli mutants with temperature-sensitive synthesis of DNA.具有温度敏感型DNA合成能力的大肠杆菌突变体。
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