Horton J E, Oppenheim J J, Mergenhagen S E
Clin Exp Immunol. 1973 Mar;13(3):383-93.
Leucocyte cultures from subjects with periodontal disease when stimulated by human dental-plaque deposit material, or phytohaemagglutinin, produce a soluble factor, lymphotoxin, which is cytotoxic for fibroblasts . The cytotoxic effect was determined from the degree of inhibition of incorporation of C-labelled L-leucine by cultures of human gingival or mouse L-fibroblasts exposed to supernatants from such cultures. Inhibition of protein synthesis by the fibroblasts was not due to either depletion of nutrients or direct toxicity of the antigenic dental-plaque material. Both plaque-stimulated leucocyte culture supernatants from clinically normal subjects and unstimulated leucocyte culture supernatants from subjects with periodontal disease were significantly less inhibitory than supernatants of plaque-stimulated leucocyte cultures from subjects with periodontal disease. This production of lymphotoxin by leucocytes stimulated with antigen(s) present in dental plaque-deposits may reflect a mechanism of tissue destruction by sensitized lymphocytes present in the tissues of subjects with periodontal disease.
来自牙周病患者的白细胞培养物在受到人牙菌斑沉积物质或植物血凝素刺激时，会产生一种可溶性因子——淋巴毒素，它对成纤维细胞具有细胞毒性。细胞毒性作用是通过将人牙龈或小鼠L - 成纤维细胞培养物暴露于此类培养物的上清液后，测定其对C标记的L - 亮氨酸掺入的抑制程度来确定的。成纤维细胞蛋白质合成的抑制并非由于营养物质的耗尽或抗原性牙菌斑物质的直接毒性。临床正常受试者的牙菌斑刺激白细胞培养物上清液和牙周病患者的未刺激白细胞培养物上清液的抑制作用均明显低于牙周病患者的牙菌斑刺激白细胞培养物上清液。牙菌斑沉积物中存在的抗原刺激白细胞产生淋巴毒素，这可能反映了牙周病患者组织中致敏淋巴细胞破坏组织的一种机制。
